Bai Yu, Bai Jia-Ming, Li Jing, Li Min, Yu Ran, Pan Qun-Wan
Department of Computer; Department of Physiology, Wannan Medical College, Wuhu 241000, China.
Sheng Li Xue Bao. 2014 Dec 25;66(6):675-82.
The purpose of the present study is to analyze the relationship between the telemetry electroencephalogram (EEG) changes of the prelimbic (PL) cortex and the drug-seeking behavior of morphine-induced conditioned place preference (CPP) rats by using the wavelet packet extraction and entropy measurement. The recording electrode was stereotactically implanted into the PL cortex of rats. The animals were then divided randomly into operation-only control and morphine-induced CPP groups, respectively. A CPP video system in combination with an EEG wireless telemetry device was used for recording EEG of PL cortex when the rats shuttled between black-white or white-black chambers. The telemetry recorded EEGs were analyzed by wavelet packet extraction, Welch power spectrum estimate, normalized amplitude and Shannon entropy algorithm. The results showed that, compared with operation-only control group, the left PL cortex's EEG of morphine-induced CPP group during black-white chamber shuttling exhibited the following changes: (1) the amplitude of average EEG for each frequency bands extracted by wavelet packet was reduced; (2) the Welch power intensity was increased significantly in 10-50 Hz EEG band (P < 0.01 or P < 0.05); (3) Shannon entropy was increased in β, γ₁, and γ₂waves of the EEG (P < 0.01 or P < 0.05); and (4) the average information entropy was reduced (P < 0.01). The results suggest that above mentioned EEG changes in morphine-induced CPP group rat may be related to animals' drug-seeking motivation and behavior launching.
本研究旨在通过小波包提取和熵测量，分析前边缘（PL）皮质的遥测脑电图（EEG）变化与吗啡诱导的条件性位置偏爱（CPP）大鼠觅药行为之间的关系。将记录电极立体定向植入大鼠的PL皮质。然后将动物随机分为仅手术对照组和吗啡诱导的CPP组。当大鼠在黑白或白黑箱之间穿梭时，使用CPP视频系统结合EEG无线遥测设备记录PL皮质的EEG。通过小波包提取、韦尔奇功率谱估计、归一化幅度和香农熵算法对遥测记录的EEG进行分析。结果表明，与仅手术对照组相比，吗啡诱导的CPP组大鼠在黑白箱穿梭期间左侧PL皮质的EEG表现出以下变化：（1）小波包提取的各频段平均EEG幅度降低；（2）10 - 50 Hz EEG频段的韦尔奇功率强度显著增加（P < 0.01或P < 0.05）；（3）EEG的β、γ₁和γ₂波中的香农熵增加（P < 0.01或P < 0.05）；（4）平均信息熵降低（P < 0.01）。结果表明，吗啡诱导的CPP组大鼠上述EEG变化可能与动物的觅药动机和行为启动有关。
