Salem Nidá, Golino Deborah A, Falk Bryce W, Rowhani Adib
Department of Plant Pathology, University of California, Davis 95616.
Plant Dis. 2008 Apr;92(4):508-512. doi: 10.1094/PDIS-92-4-0508.
A number of viruses in the genera Ilarvirus and Nepovirus have been shown to be associated with specific diseases in rose, but many graft-transmissible rose diseases still have unknown etiologies. One of these diseases originally was detected by grafting from nonsymptomatic roses to Rosa multiflora indicator plants. Double-stranded RNAs (dsRNAs) were recovered and used as templates for cDNA synthesis and generating a cDNA library. Analysis of deduced amino acid sequences clearly positioned this virus as a member of the family Luteoviridae. The name rose spring dwarf associated virus (RSDaV) is tentatively proposed for the novel virus because the symptoms of this virus on R. multiflora are consistent with previous descriptions of rose spring dwarf disease (RSD). Phylogenetic analysis revealed a close relationship of RSDaV with members of the genus Luteovirus. Aphid transmission studies identified the rose-grass aphid (Metapolophium dirhodum) and yellow rose aphid (Rhodobium porosum) as vectors for this new virus. Host range data showed that RSDaV has a host range including both monocots and dicots. A specific reverse-transcription polymerase chain reaction assay was developed and revealed the presence of the RSDaV in several rose cultivars. RSDaV-inoculated rose plants developed RSD symptoms, confirming its role in the etiology of the disease.
已证明一些等轴不稳环斑病毒属和线虫传多面体病毒属的病毒与玫瑰中的特定病害有关，但许多可通过嫁接传播的玫瑰病害的病因仍不明。这些病害之一最初是通过将无症状玫瑰嫁接到多花蔷薇指示植物上检测到的。回收双链RNA（dsRNA）并将其用作cDNA合成和构建cDNA文库的模板。对推导的氨基酸序列的分析明确将这种病毒定位为黄症病毒科的成员。由于这种病毒在多花蔷薇上的症状与先前对玫瑰春季矮缩病（RSD）的描述一致，因此暂定将这种新型病毒命名为玫瑰春季矮缩相关病毒（RSDaV）。系统发育分析显示RSDaV与黄症病毒属成员关系密切。蚜虫传播研究确定玫瑰禾谷缢管蚜（Metapolophium dirhodum）和黄玫瑰蚜（Rhodobium porosum）是这种新病毒的传播媒介。寄主范围数据表明RSDaV的寄主范围包括单子叶植物和双子叶植物。开发了一种特异性逆转录聚合酶链反应检测方法，并揭示了几种玫瑰品种中存在RSDaV。接种RSDaV的玫瑰植株出现了RSD症状，证实了其在该病病因中的作用。
