Department of Neurology and the Multiple Sclerosis Center, the Agnes-Ginges Center for Neurogenetics, Hadassah- Medical Center, Hebrew University, Ein Karem, P.O.B. 12000, Jerusalem 91120, Israel.
Department of Neurology and the Multiple Sclerosis Center, the Agnes-Ginges Center for Neurogenetics, Hadassah- Medical Center, Hebrew University, Ein Karem, P.O.B. 12000, Jerusalem 91120, Israel; Leslie and Michael Center for Neuro-oncology, Hadassah- Medical Center, Jerusalem, Israel.
Mult Scler Relat Disord. 2019 May;30:114-118. doi: 10.1016/j.msard.2019.01.047. Epub 2019 Jan 30.
Alterations in the frequency or function of regulatory T cells (Tregs), which play a critical role in the maintenance of peripheral immune tolerance, are known to be involved in the pathogenesis of several autoimmune diseases. Neuromyelitis optica spectrum disorders (NMOSD) are autoimmune inflammatory diseases of the central nervous system (CNS), of which the etiology and mechanisms underlying its development are not completely understood. Although there is increasing evidence for the involvement of effector T cells in NMOSD, no data are available regarding the role of Tregs in its pathogenesis.
The aim of this study was to investigate the mRNA expression level of regulatory T cell genes in NMOSD.
We used gene expression array and RT-PCR analysis to study Treg cell genes in NMOSD RESULTS: A distinctive Treg gene signature in the peripheral blood of NMOSD patients is described, as well as significantly decreased FoxP3 mRNA expression in the peripheral blood mononuclear cells (PBMCs) of the patients vs that in the healthy controls (HCs) (NMOSD,1.8RQ vs HC, 6.8RQ, p = 0.01).
The present study shows downregulation at the mRNA expression level of a Treg key transcription factor FoxP3, in NMOSD. Exploration of Tregs function and interconnections in the peripheral immune system should advance our understanding of NMOSD pathogenesis.
调节性 T 细胞（Tregs）的频率或功能的改变，在维持外周免疫耐受中起着关键作用，已知与几种自身免疫性疾病的发病机制有关。视神经脊髓炎谱系疾病（NMOSD）是一种中枢神经系统（CNS）自身免疫性炎症性疾病，其病因和发病机制尚不完全清楚。尽管越来越多的证据表明效应 T 细胞参与了 NMOSD，但关于 Tregs 在其发病机制中的作用尚无数据。
本研究旨在研究 NMOSD 中调节性 T 细胞基因的 mRNA 表达水平。
我们使用基因表达谱和 RT-PCR 分析研究了 NMOSD 中的 Treg 细胞基因。
描述了 NMOSD 患者外周血中的独特 Treg 基因特征，以及患者外周血单核细胞（PBMCs）中 FoxP3 mRNA 表达明显低于健康对照组（HCs）（NMOSD，1.8RQ 与 HC，6.8RQ，p=0.01）。
本研究显示 NMOSD 中 Treg 关键转录因子 FoxP3 的 mRNA 表达水平下调。探索外周免疫系统中 Tregs 的功能和相互联系应能增进我们对 NMOSD 发病机制的理解。
