巴西圈养鹦形目鸟类中贾第虫属的检测与分子特征分析
Detection and molecular characterization of Giardia spp. in captive Psittaciformes in Brazil.
作者信息
Ichikawa Ricardo Shoiti, Santana Bruna Nicoleti, Ferrari Elis Domingos, do Nascimento Isabela Garcia, Nakamura Alex Akira, Nardi Ana Rita Moraes, Meireles Marcelo Vasconcelos
机构信息
Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatuba. Rua Clóvis Pestana, 793, CEP 16050-680, Bairro Dona Amélia, Araçatuba, SP, Brazil.
Fundação Municipal de Ensino Superior, Bragança Paulista, São Paulo, Brazil.
出版信息
Prev Vet Med. 2019 Mar 1;164:10-12. doi: 10.1016/j.prevetmed.2019.01.006. Epub 2019 Jan 15.
This study aimed to perform the detection and molecular characterization of Giardia spp. in Psittaciformes from the Southern and Southeastern regions of Brazil. Fecal samples were obtained from 359 adult exotic captive Psittaciformes belonging to 13 genera, randomly selected from 33 aviaries located in the Southern and Southeastern regions of Brazil during a bird exhibition at the 2015 Ornithological Championship of the Ornithological Federation of Brazil (FOB). Nested polymerase chain reaction targeting the small subunit rRNA gene identified Giardia spp. in 93/359 (25.9%) fecal samples and 25/33 (75.8%) aviaries. Genetic sequencing identified G. psittaci in 12 birds from six genera. Zoonotic Giardia species was not detected in fecal samples from Psittaciformes.
本研究旨在对巴西南部和东南部地区鹦鹉目鸟类中的贾第虫属进行检测和分子特征分析。在2015年巴西鸟类学联合会（FOB）鸟类锦标赛的鸟类展览期间，从巴西南部和东南部地区33个鸟舍中随机选取了359只属于13个属的成年外来圈养鹦鹉目鸟类，采集其粪便样本。针对小亚基rRNA基因的巢式聚合酶链反应在93/359（25.9%）的粪便样本和25/33（75.8%）的鸟舍中鉴定出贾第虫属。基因测序在来自六个属的12只鸟类中鉴定出鹦鹉贾第虫。在鹦鹉目鸟类的粪便样本中未检测到人畜共患的贾第虫物种。
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