德国鹦鹉中鹦鹉贾第虫的发生情况及分子分型——一项案例研究

Occurrence and Molecular Typing of Giardia psittaci in Parakeets in Germany-A Case Study.

作者信息

Reuschel M, Pantchev N, Vrhovec M Globokar, Jung A, Gerhauser I, Sannella A R, Cacciò S M, Legler M

机构信息

Clinic for Small Mammals, Reptiles, and Birds, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany,

IDEXX Laboratories, Mörikestraße 28/3, 71636 Ludwigsburg, Germany.

出版信息

Avian Dis. 2020 Jun;64(2):228-233. doi: 10.1637/0005-2086-64.2.228.

DOI:10.1637/0005-2086-64.2.228

PMID:32550625

Abstract

A grey-hooded parakeet () and two budgerigars () from different owners presented with decreased activity, vomitus, and diarrhea. A microscopic examination of feces showed trophozoites of the protozoan flagellate . A commercial immunochromatographic dipstick test for sp. antigens confirmed the infection. These findings were assured by PCR of the small subunit ribosomal RNA (SSU rRNA) gene and coproantigen ELISA. Sequencing of PCR products of the SSU rRNA (292 bp) and β-giardin genes (511 bp) identified as the species involved. Therefore, our results show that a GSA 65-based coproantigen ELISA, which was established for diagnosis of is applicable for the detection of . A treatment with ronidazole was started. Additionally, fecal examination and dissection of the dead birds revealed coinfection with the fungal pathogen . One budgerigar survived and repeatedly tested negative after treatment with ronidazole. The described cases indicate that a single infection with has a good prognosis, whereas the prognosis is poor when coinfections occur, especially with .

摘要

一只来自不同主人的灰头鹦鹉（）和两只虎皮鹦鹉（）出现活动减少、呕吐和腹泻症状。粪便显微镜检查显示有原生动物鞭毛虫的滋养体。针对属物种抗原的商业免疫层析试纸条检测证实了感染。这些结果通过小亚基核糖体RNA（SSU rRNA）基因的PCR和粪抗原ELISA得以确认。对SSU rRNA（292 bp）和β-贾第素基因（511 bp）的PCR产物进行测序确定所涉及的物种为。因此，我们的结果表明，为诊断而建立的基于GSA 65的粪抗原ELISA适用于的检测。开始使用甲硝唑进行治疗。此外，对死鸟的粪便检查和解剖显示同时感染了真菌病原体。一只虎皮鹦鹉存活下来，用甲硝唑治疗后多次检测呈阴性。所述病例表明，单一感染预后良好，而发生合并感染时，尤其是合并感染时，预后较差。