Costa Aristóteles G, de Carvalho Antônio Último, Melo Marília M, Soto-Blanco Benito
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos 6627, Belo Horizonte, MG, 30123-970, Brazil.
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos 6627, Belo Horizonte, MG, 30123-970, Brazil.
Toxicon. 2019 Mar 15;160:8-11. doi: 10.1016/j.toxicon.2019.02.005. Epub 2019 Feb 15.
Monofluoroacetate (MFA) is considered one of the most toxic substances known. It is found naturally in plants, and causes sudden death syndrome in ruminants. Due to hyperacute evolution of poisoning and the absence of effective treatment, induction of resistance in animals might be the best tool to control MFA poisoning in ruminants. The objective of this study was to promote resistance in cattle against the toxic effects of MFA through its degradation by the ruminal microbiota after the administration of sodium trifluoroacetate (TFA). Ten calves were distributed into two groups: control group (n = 3) and treated group (n = 7). The calves in the treated group received 0.1 mg/kg live weight of TFA, whereas, those in the control group received water; both for 28 consecutive days. The calves were subjected to daily clinical evaluation and weekly blood biochemical determination to identify any signs of poisoning. After 28 d of administration of TFA or water, 2.0 g/kg body weight of Palicourea marcgravii leaves (containing 0.15% MFA) were administered using a stomach tube to determine the occurrence of resistance. The administration of TFA did not induce any clinical or biochemical changes in blood. The administration of P. marcgravii induced clinical changes in the calves of control group, but there was no change in the calves of the treated group. In conclusion, the administration of TFA to cattle can induce effective resistance against MFA poisoning.
一氟乙酸盐（MFA）被认为是已知毒性最强的物质之一。它天然存在于植物中，可导致反刍动物猝死综合征。由于中毒发展迅速且缺乏有效治疗方法，诱导动物产生抗性可能是控制反刍动物MFA中毒的最佳手段。本研究的目的是通过在给牛投喂三氟乙酸钠（TFA）后，利用瘤胃微生物群对其进行降解，从而增强牛对MFA毒性作用的抗性。将10头小牛分为两组：对照组（n = 3）和处理组（n = 7）。处理组的小牛接受0.1mg/kg体重的TFA，而对照组的小牛接受水；均连续投喂28天。对小牛进行每日临床评估和每周血液生化测定，以确定是否有中毒迹象。在投喂TFA或水28天后，通过胃管给小牛投喂2.0g/kg体重的马氏九里香树叶（含0.15% MFA），以确定抗性的发生情况。投喂TFA未引起血液中的任何临床或生化变化。投喂马氏九里香树叶在对照组小牛中引起了临床变化，但处理组小牛未出现变化。总之，给牛投喂TFA可诱导对MFA中毒的有效抗性。
