da Silva Layze C A, Pessoa Danielle A N, Lopes Jose R G, de Albuquerque Laio G, da Silva Leomyr S A, Garino Junior Felicio, Riet-Correa Franklin
Veterinary Hospital Federal University of Campina Grande, Patos, Paraiba, CEP 58700-000, Brazil.
Veterinary Hospital Federal University of Campina Grande, Patos, Paraiba, CEP 58700-000, Brazil; National Institute of Agricultural Research (INIA), La Estanzuela, Casilla de correo, 68033, Colonia, Uruguay.
Toxicon. 2016 Mar 1;111:65-8. doi: 10.1016/j.toxicon.2015.12.016. Epub 2015 Dec 30.
The intraruminal inoculation of sodium monofluoroacetate (MFA)-degrading bacteria has been proposed as a method to prevent poisoning by MFA-containing plants. In previous experiments, MFA-degrading bacteria were inoculated intraruminally before or concurrent with plant challenge, with both strategies conferring partial protection to poisoning. To evaluate the protection to Amorimia septentrionalis poisoning provided by the continuous inoculation of MFA-degrading bacteria isolated from plants and soils, 18 goats were divided into three experimental groups of six animals each: Group 1 goats received daily doses of a mixture of Paenibacillus sp. and Cupriavidus sp., and Group 2 goats received a mixture of Ralstonia sp. and Burkholderia sp., for 40 days, while Group 3 goats were not inoculated. Ten days after initiation of bacterial inoculation in Groups 1 and 2, all goats were challenged daily with 5 g/kg body weight of green leaves from A. septentrionalis. Four goats from Group 1 consumed the leaves throughout the 30-day consumption period and showed clinical signs such as transient tachycardia and engorgement of the jugular. The two remaining animals from Group 1 showed obvious signs of intoxication, and plant administration was suspended on days 17 and 19. The goats in Group 2 consumed the leaves throughout the 30-day study without showing signs of poisoning. The goats from Group 3 (control) manifested severe clinical signs of poisoning between the 3rd and 10th days following the start of the A. septentrionalis challenge. Under the conditions of this experiment continuous intraruminal administration of Ralstonia sp. and Burkholderia sp. provided complete protection to poisoning by A. septentrionalis in goats, while continuous intraruminal administration of Paenibacillus sp. and Cupriavidus sp. provided partial protection.
瘤胃内接种单氟乙酸钠（MFA）降解菌已被提议作为一种预防采食含MFA植物中毒的方法。在之前的实验中，MFA降解菌在植物攻毒前或同时进行瘤胃内接种，两种策略均能对中毒起到部分保护作用。为了评估从植物和土壤中分离出的MFA降解菌持续接种对北方紫堇中毒的保护作用，将18只山羊分为三个实验组，每组6只动物：第1组山羊每天接受芽孢杆菌属和贪铜菌属混合物的剂量，第2组山羊接受雷尔氏菌属和伯克霍尔德菌属混合物的剂量，持续40天，而第3组山羊不接种。在第1组和第2组开始细菌接种10天后，所有山羊每天用5 g/kg体重的北方紫堇绿叶进行攻毒。第1组的4只山羊在整个30天的采食期内食用了这些叶子，并表现出短暂心动过速和颈静脉充血等临床症状。第1组剩下的2只动物表现出明显的中毒迹象，在第17天和第19天停止投喂植物。第2组的山羊在整个30天的研究中食用了这些叶子，没有表现出中毒迹象。第3组（对照组）的山羊在北方紫堇攻毒开始后的第3天至第10天之间表现出严重的中毒临床症状。在本实验条件下，瘤胃内持续接种雷尔氏菌属和伯克霍尔德菌属能为山羊提供完全的北方紫堇中毒保护，而瘤胃内持续接种芽孢杆菌属和贪铜菌属则提供部分保护。
