Lv Dongling, Chen Xiaohu, Zhang Nongshan, Wu Xiang, Jiang Meng
Department of Cardiovascular Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, The No.1 Clinical Medical College of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, China.
Pak J Pharm Sci. 2019 Jan;32(1):1-5.
Total flavones of Abelmoschus manihot L. Medic (TFA) is the major active component isolated from Chinese herb Abelmoschus manihot L. Medic. TFA has shown neuroprotective effect against cerebral ischemia injury in rats and rabbits. However, the effects of TFA on hind-limb ischemia and the underlying mechanisms remain unclear. Therefore, in the present study, we used a rat hind-limb ischemia model to investigate protective effect of TFA against limb ischemia injury. The rat model of hind-limb ischemia was established. Treatment groups received TFA at two different doses (160 and 40mg/kg) daily for 10 days. Sham operated control group and model group received saline. At the end the rats were sacrificed, hindlimb tissues were stained with Haematoxylin-Eosin and Masson's trichrome. RNA and protein were extracted from tissues for PCR and Western blot analysis. The results showed that TFA reduced lower limb ischemic injury, recovered tissue volume and diminished fibrosis and muscle degeneration. Mechanistically, we showed that TFA increased the expression of anti-apoptotic factor such as Bcl-2 and survivin, decreased the expression of pro-apoptotic factor such as Caspase 3, Bax and Bak and inhibited the activation of caspase 3 and 9. In summary, this study proves new evidence that TFA protects hind-limb against ischemia injury by inhibiting apoptosis and could be a promising therapeutic agent for acute lower extremity ischemia.
黄蜀葵花总黄酮（TFA）是从中药黄蜀葵中分离得到的主要活性成分。TFA已显示出对大鼠和家兔脑缺血损伤具有神经保护作用。然而，TFA对后肢缺血的影响及其潜在机制仍不清楚。因此，在本研究中，我们使用大鼠后肢缺血模型来研究TFA对肢体缺血损伤的保护作用。建立大鼠后肢缺血模型。治疗组每天接受两种不同剂量（160和40mg/kg）的TFA，持续10天。假手术对照组和模型组接受生理盐水。在实验结束时处死大鼠，后肢组织用苏木精-伊红和马松三色染色。从组织中提取RNA和蛋白质用于PCR和蛋白质印迹分析。结果表明，TFA减轻了下肢缺血损伤，恢复了组织体积，减少了纤维化和肌肉变性。从机制上讲，我们发现TFA增加了抗凋亡因子如Bcl-2和survivin的表达，降低了促凋亡因子如Caspase 3、Bax和Bak的表达，并抑制了Caspase 3和9的激活。总之，本研究证明了新的证据，即TFA通过抑制细胞凋亡保护后肢免受缺血损伤，可能是治疗急性下肢缺血的有前途的治疗剂。
