褪黑素治疗对慢性应激诱导抑郁大鼠模型肾上腺儿茶酚胺生物合成酶和去甲肾上腺素转运体基因表达变化的影响。
Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression.
机构信息
a Department of Molecular Biology and Endocrinology, Institute of Nuclear Sciences "Vinca", University of Belgrade, Belgrade, Serbia.
b CEDARS-SINAI, Center for Neural Science and Medicine, 8700 Beverly Blvd., Los Angeles, CA 90048, USA.
出版信息
Can J Physiol Pharmacol. 2019 Jul;97(7):685-690. doi: 10.1139/cjpp-2018-0612. Epub 2019 Feb 16.
This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine -methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression.
本研究探讨了褪黑素治疗对慢性不可预测轻度应激（CUMS）抑郁模型大鼠肾上腺儿茶酚胺含量、合成、摄取和囊泡转运的影响。这需要定量测定去甲肾上腺素、肾上腺素、酪氨酸羟化酶 (TH)、多巴胺-β-羟化酶 (DBH)、苯乙醇胺-N-甲基转移酶 (PNMT)、去甲肾上腺素转运体 (NET) 和囊泡单胺转运体 2 (VMAT2) 的 mRNA 和蛋白水平在肾上腺髓质中。CUMS 导致去甲肾上腺素储存和 TH、DBH 和 NET 的蛋白水平显著减少，而 PNMT 的基因表达增加。观察到褪黑素治疗可预防 CUMS 大鼠的应激诱导的去甲肾上腺素含量降低以及慢性应激大鼠肾上腺髓质中 TH、DBH 和 NET 的蛋白表达降低。本研究表明褪黑素对大鼠慢性应激诱导抑郁模型中肾上腺髓质儿茶酚胺合成、摄取和含量具有刺激作用。
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