He Li-Wen, Zeng Li, Tian Na, Li Yi, He Tong, Tan Dong-Mei, Zhang Qian, Tan Yi
Laboratory Animal Center Chongqing Medical University Chongqing China.
Pediatric Research Institute Children's Hospital of Chongqing Medical University Chongqing China.
Animal Model Exp Med. 2020 Mar 31;3(1):69-78. doi: 10.1002/ame2.12107. eCollection 2020 Mar.
The chronic unpredictable mild stress (CUMS) model has long been considered the best model for exploring the pathophysiological mechanisms underlying depression. However, there are no widely recognised standards for strategies for modeling and for behavioral testing. The present study aimed to optimize the protocols for food deprivation and the sucrose preference test (SPT) for the CUMS model.
We first evaluated the effects of different long periods of food deprivation on the body weight of Sprague Dawley (SD) rats by testing food deprivation for 24 hours (8:00-8:00), food deprivation for 12 hours during the daytime (8:00-20:00) and food deprivation for 12 hours at night (20:00-8:00). Next, we established a SD rat CUMS model with 15 different stimulations, and used body weight measurement, SPT, forced swim test (FST), open field test (OFT) and Morris water maze (MWM) test to verify the success of the modeling. In the SPT, consumption of sucrose and pure water within 1 and 12 hours was measured.
Twelve hours of food deprivation during the daytime (8:00-20:00) had no effect on body weight, while 12 hours of food deprivation at night (20:00-8:00) and 24 hours of food deprivation (8:00-8:00) significantly reduced the mean body weight of the SD rats. When SPT was used to verify the successful establishment of the CUMS rat model, sucrose consumption measured within 12 hours was less variable than that measured within 1 hour.
Twelve hours of food deprivation in the daytime (8:00-20:00) may be considered a mild stimulus for the establishment of a CUMS rat model. Measuring sucrose consumption over 12 hours is recommended for SPT.
长期以来，慢性不可预测轻度应激（CUMS）模型一直被认为是探索抑郁症潜在病理生理机制的最佳模型。然而，目前尚无关于建模策略和行为测试的广泛认可的标准。本研究旨在优化CUMS模型的禁食方案和蔗糖偏好试验（SPT）。
我们首先通过测试24小时禁食（8:00-8:00）、白天12小时禁食（8:00-20:00）和夜间12小时禁食（20:00-8:00），评估不同长时间禁食对Sprague Dawley（SD）大鼠体重的影响。接下来，我们建立了一个包含15种不同刺激的SD大鼠CUMS模型，并使用体重测量、SPT、强迫游泳试验（FST）、旷场试验（OFT）和莫里斯水迷宫（MWM）试验来验证建模的成功。在SPT中，测量1小时和12小时内蔗糖和纯水的消耗量。
白天12小时禁食（8:00-20:00）对体重没有影响，而夜间12小时禁食（20:00-8:00）和24小时禁食（8:00-8:00）显著降低了SD大鼠的平均体重。当使用SPT验证CUMS大鼠模型的成功建立时，12小时内测量的蔗糖消耗量比1小时内测量的更稳定。
白天12小时禁食（8:00-20:00）可被视为建立CUMS大鼠模型的轻度刺激。建议在SPT中测量12小时内的蔗糖消耗量。
