Noormohammadi Amir H, Whithear Kevin G
a School of Veterinary and Agricultural Sciences , Werribee , Australia.
Avian Pathol. 2019 Jun;48(3):238-244. doi: 10.1080/03079457.2019.1572103. Epub 2019 Feb 18.
In order to compare the short-term efficacies of the live attenuated Mycoplasma gallisepticum (MG) vaccine strains ts-11 and 6/85, four groups of SPF chickens were vaccinated with each of the vaccines using eye drop and aerosol inoculations, and were subsequently challenged with a wild-type MG strain. When administered by the recommended routes (eye drop for ts-11 and fine aerosol for 6/85), both vaccines induced substantial and comparable levels of protection against airsacculitis and tracheitis caused by wild-type MG. The long-term efficacies of the two vaccines administered by the recommended route were also assessed. Serum antibody responses and colonization of the vaccines in the upper respiratory system were monitored at different time points after vaccination, and protective efficacies of the vaccines were evaluated at 36 weeks post vaccination as above. Systemic antibody response following ts-11 eye drop vaccination was initially strong but reduced gradually over time while, in contrast, that to 6/85 spray vaccination was initially weak but increased over time. Kinetics of the antibody response to the vaccines appeared to be correlated with the number of birds harbouring each vaccine in their upper respiratory system throughout the sampling timepoints. Regardless of the levels of serum antibodies or number of birds harbouring the vaccine, both vaccines induced substantial and comparable levels of protection against airsacculitis and tracheitis caused by wild-type MG. Therefore, kinetics of systemic antibody response and persistence in the upper respiratory system varies between vaccine strains; however, the levels of protection may not, at least up to 36 weeks post vaccination. RESEARCH HIGHLIGHTS The kinetics of systemic antibody response and persistence of the vaccine in the upper respiratory system varies between vaccine strains ts-11 and 6/85. The levels of protection induced by the two vaccines against virulent MG strain challenge are comparable when they are administered by the route recommended by their manufacturers.
为比较减毒活鸡毒支原体（MG）疫苗株ts - 11和6/85的短期效力，将四组SPF鸡分别通过滴眼和气雾接种的方式接种每种疫苗，随后用野生型MG菌株进行攻毒。当按照推荐途径给药时（ts - 11滴眼，6/85精细喷雾），两种疫苗均诱导产生了针对野生型MG引起的气囊炎和气管炎的高水平且相当的保护作用。还评估了按推荐途径给药的两种疫苗的长期效力。在接种疫苗后的不同时间点监测血清抗体反应以及疫苗在上呼吸道系统中的定植情况，并在接种后36周如上述那样评估疫苗的保护效力。ts - 11滴眼接种后的全身抗体反应最初较强，但随时间逐渐降低，而相比之下，6/85喷雾接种后的全身抗体反应最初较弱，但随时间增加。在整个采样时间点，对疫苗的抗体反应动力学似乎与上呼吸道系统中携带每种疫苗的鸡的数量相关。无论血清抗体水平或携带疫苗的鸡的数量如何，两种疫苗均诱导产生了针对野生型MG引起的气囊炎和气管炎的高水平且相当的保护作用。因此，不同疫苗株之间全身抗体反应的动力学以及在上呼吸道系统中的持续存在情况有所不同；然而，至少在接种后36周，保护水平可能并无差异。研究亮点不同疫苗株ts - 11和6/85之间全身抗体反应的动力学以及疫苗在上呼吸道系统中的持续存在情况有所不同。当按照制造商推荐的途径给药时，两种疫苗针对强毒MG菌株攻毒诱导的保护水平相当。
