Kulappu Arachchige Sathya N, Young Neil D, Kanci Condello Anna, Omotainse Oluwadamilola S, Noormohammadi Amir H, Wawegama Nadeeka K, Browning Glenn F
Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia.
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia.
Front Immunol. 2021 Feb 2;11:628804. doi: 10.3389/fimmu.2020.628804. eCollection 2020.
Live attenuated vaccines are commonly used to control infections in chickens. ts-304 is a novel live attenuated vaccine strain that has been shown to be safe and effective. In this study, the transcriptional profiles of genes in the tracheal mucosa in chickens challenged with the wild-type strain Ap3AS at 57 weeks after vaccination with ts-304 were explored and compared with the profiles of unvaccinated chickens that had been challenged with strain Ap3AS, unvaccinated and unchallenged chickens, and vaccinated but unchallenged chickens. At two weeks after challenge, pair-wise comparisons of transcription in vaccinated-only, vaccinated-and-challenged and unvaccinated and unchallenged birds detected no differences. However, the challenged-only birds had significant up-regulation in the transcription of genes and enrichment of gene ontologies, pathways and protein classes involved in infiltration and proliferation of inflammatory cells and immune responses mediated through enhanced cytokine and chemokine production and signaling, while those predicted to be involved in formation and motor movement of cilia and formation of the cellular cytoskeleton were significantly down-regulated. The transcriptional changes associated with the inflammatory response were less severe in these mature birds than in the relatively young birds examined in a previous study. The findings of this study demonstrated that vaccination with the attenuated strain ts-304 protects against the transcriptional changes associated with the inflammatory response and pathological changes in the tracheal mucosa caused by infection with in chickens for at least 57 weeks after vaccination.
减毒活疫苗常用于控制鸡的感染。ts-304是一种新型减毒活疫苗株，已被证明安全有效。在本研究中，探讨了用ts-304疫苗接种57周后，用野生型菌株Ap3AS攻击的鸡气管黏膜中基因的转录谱，并与未接种疫苗但用Ap3AS菌株攻击的鸡、未接种疫苗且未受攻击的鸡以及接种疫苗但未受攻击的鸡的转录谱进行了比较。攻击后两周，仅接种疫苗、接种疫苗并受攻击以及未接种疫苗且未受攻击的鸡之间的转录两两比较未发现差异。然而，仅受攻击的鸡在基因转录方面有显著上调，并且在炎症细胞浸润和增殖以及通过增强细胞因子和趋化因子产生及信号传导介导的免疫反应所涉及的基因本体、途径和蛋白质类别方面有富集，而那些预计参与纤毛形成和运动以及细胞骨架形成的基因则显著下调。与先前研究中检查的相对年轻的鸡相比，这些成熟鸡中与炎症反应相关的转录变化不那么严重。本研究结果表明，用减毒株ts-304接种疫苗可预防接种后至少57周内鸡感染引起的气管黏膜炎症反应相关转录变化和病理变化。
