Cai Kai, Zhu Longfei, Zhang Keke, Li Ling, Zhao Zhongyu, Zeng Wei, Lin Xinchun
Sino-Australia Plant Cell Wall Research Centre, State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an, China.
Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-Efficiency Utilization, Zhejiang A & F University, Lin'an, China.
Front Plant Sci. 2019 Feb 1;10:50. doi: 10.3389/fpls.2019.00050. eCollection 2019.
Bamboo are woody grass species containing important economic and ecological values. Lei bamboo () is a kind of shoot-producing bamboo species with the highest economic yield per unit area. However, identifying different varieties of Lei bamboo based on morphological characteristics is difficult. Microsatellites play an important role in plant identification and genetic diversity analysis and are superior to other molecular markers. In this study, we identified 18,356 expressed sequence tag-simple sequence repeat (EST-SSR) loci in Lei bamboo transcriptome data. A total of 11,264 primer pairs were successfully designed from unigenes of all EST-SSR loci, and 96 primer pairs were randomly selected and synthesized. A total of 54 primer pairs were used for classifying 16 Lei bamboo varieties and 10 different species. The number of polymorphism alleles among the 54 primer pairs ranged from 3 to 12 for varieties and 3 to 20 for . The phylogenetic tree based on polymorphism alleles successfully distinguished 16 varieties and 10 species. Our study provides abundant EST-SSR resources that are useful for genetic diversity analysis and molecular verification of bamboo and suggests that SSR markers developed from Lei bamboo are more efficient and reliable than ISSR, SRAP or AFLP markers.
竹子是具有重要经济和生态价值的木本禾本科植物。雷竹（）是单位面积经济产量最高的笋用竹种。然而，基于形态特征鉴别雷竹的不同品种很困难。微卫星在植物鉴定和遗传多样性分析中发挥着重要作用，且优于其他分子标记。在本研究中，我们在雷竹转录组数据中鉴定出18356个表达序列标签 - 简单序列重复（EST - SSR）位点。从所有EST - SSR位点的单基因成功设计出总共11264对引物，并随机选择并合成了96对引物。总共54对引物用于对16个雷竹品种和10个不同竹种进行分类。54对引物中多态性等位基因的数量，雷竹品种为3至12个，其他竹种为3至20个。基于多态性等位基因构建的系统发育树成功区分了16个雷竹品种和10个竹种。我们的研究提供了丰富的EST - SSR资源，可用于竹子的遗传多样性分析和分子验证，并表明从雷竹开发的SSR标记比ISSR、SRAP或AFLP标记更有效、更可靠。
