Chromosome-level genome assembly and annotation of Phyllostachys violascens 'Prevernalis'.

Phyllostachys violascens 'Prevernalis' is one of the best species for shoots, and the Prevernalis shoots are widely favored by people for its high nutritional value, high medicinal value and delicious taste. However, high-quality reference genome for Prevernalis has not been published, primarily due to its polyploidy and large genome size. Here, we generated a high-quality genome for tetraploid Prevernalis using a combination of PacBio long reads and Hi-C sequencing techniques. The final genome length of Prevernalis is 2156.14 Mb, with a scaffold N50 size of 56.48 Mb, a BUSCO score of 97.51% and LAI score of 20.03. The assembled genome comprises 68.45% repetitive elements, and was anchored to twenty-four chromosomes. Moreover, we identified a total of 53,558 protein-coding genes, of which 94.13% were functionally annotated. Furthermore, comparing the genome of Prevernalis with seven other species showed that the gene numbers and structures of Prevernalis are similar with Ph.edulis. Overall, the high-quality genome assembly and gene annotation resources will provide crucial resources for functional genomic studies and molecular breeding in Prevernalis.