Wang Haixia, Ding Hui, Cheng Ping, Jin Xianjiang, Fu Yanru, Peng Yizhen
Jiangxi Academy of Forestry, Jiangxi, 330013, China.
Jiangxi Provincial Key Laboratory of Improved Variety Breeding and Efficient Utilization of Native Tree Species, Jiangxi, 330013, China.
Sci Data. 2025 May 30;12(1):912. doi: 10.1038/s41597-025-04556-1.
Phyllostachys violascens 'Prevernalis' is one of the best species for shoots, and the Prevernalis shoots are widely favored by people for its high nutritional value, high medicinal value and delicious taste. However, high-quality reference genome for Prevernalis has not been published, primarily due to its polyploidy and large genome size. Here, we generated a high-quality genome for tetraploid Prevernalis using a combination of PacBio long reads and Hi-C sequencing techniques. The final genome length of Prevernalis is 2156.14 Mb, with a scaffold N50 size of 56.48 Mb, a BUSCO score of 97.51% and LAI score of 20.03. The assembled genome comprises 68.45% repetitive elements, and was anchored to twenty-four chromosomes. Moreover, we identified a total of 53,558 protein-coding genes, of which 94.13% were functionally annotated. Furthermore, comparing the genome of Prevernalis with seven other species showed that the gene numbers and structures of Prevernalis are similar with Ph.edulis. Overall, the high-quality genome assembly and gene annotation resources will provide crucial resources for functional genomic studies and molecular breeding in Prevernalis.
雷竹‘早竹’是笋用优良竹种之一，早竹笋因其营养价值高、药用价值高、口感鲜美而深受人们喜爱。然而，早竹高质量的参考基因组尚未公布，主要原因是其多倍体特性和基因组庞大。在此，我们结合PacBio长读长测序和Hi-C测序技术，为四倍体早竹构建了高质量基因组。早竹的最终基因组长度为2156.14 Mb，支架N50大小为56.48 Mb，BUSCO评分为97.51%，LAI评分为20.03。组装的基因组包含68.45%的重复元件，并被锚定到24条染色体上。此外，我们共鉴定出53558个蛋白质编码基因，其中94.13%得到了功能注释。此外，将早竹基因组与其他7个物种进行比较，结果表明早竹的基因数量和结构与毛竹相似。总体而言，高质量的基因组组装和基因注释资源将为早竹的功能基因组学研究和分子育种提供关键资源。
