Ouryazdanpanah Noushin, Dabiri Shahriar, Derakhshani Ali, Vahidi Reza, Farsinejad Alireza
Dept. of Hematology and Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Pathol. 2018 Fall;13(4):461-466. Epub 2018 Sep 25.
The mesenchymal stem cells derived from peripheral blood (PB) have been recognized as a promising source for allogeneic cell therapy. The aim of this study was to investigate the isolation, growth and differentiation ability of peripheral blood-isolated mesenchymal stem cells.
The mononuclear cells were purified from fresh peripheral blood using density gradient centrifugation then cultured in a suitable medium, expanded and characterized. In the following, these cells were cultured in specific adipogenic and osteogenic differentiation media.
In spite of the absence of any stimulating factor, the cells adhered to the flasks and developed a rather homogeneous, spindle-shaped morphology after consecutive passages. The cells were confirmed to have mesenchymal phenotype by expression of specific markers (CD90, CD105, and CD73) and absence of CD45 marker, which is specific for hematopoietic stem cells. They could differentiate into lineage-specific committed cells (osteoblasts and adipocytes).According to the findings, the conventional, labour-intensive and time-consuming approaches are not necessary to obtain an optimal number of cells from peripheral blood. This relatively accessible and minimally invasive source of stem cells may open a new era for practical exploitation in regenerative medicine.
外周血来源的间充质干细胞已被认为是同种异体细胞治疗的一个有前景的来源。本研究的目的是探讨从外周血分离的间充质干细胞的分离、生长及分化能力。
使用密度梯度离心法从新鲜外周血中纯化单核细胞，然后在合适的培养基中培养、扩增并鉴定。接下来，将这些细胞在特定的成脂和成骨分化培养基中培养。
尽管没有任何刺激因子，细胞仍能贴壁生长，连续传代后形成相当均匀的纺锤形形态。通过特定标志物（CD90、CD105和CD73）的表达以及造血干细胞特异性标志物CD45的缺失，证实这些细胞具有间充质表型。它们能够分化为谱系特异性定向细胞（成骨细胞和脂肪细胞）。根据研究结果，从外周血中获得最佳数量的细胞并不需要传统的、劳动强度大且耗时的方法。这种相对容易获取且微创的干细胞来源可能会为再生医学的实际应用开启一个新时代。
