Trivanović Drenka, Kocić Jelena, Mojsilović Slavko, Krstić Aleksandra, Ilić Vesna, Djordjević Ivana Okić, Santibanez Juan Francisco, Jovcić Gordana, Terzić Milan, Bugarski Diana
University of Belgrade, Serbia.
Srp Arh Celok Lek. 2013 Mar-Apr;141(3-4):178-86. doi: 10.2298/sarh1304178t.
Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent.
The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton's Jelly (UC-MSCs).
The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential.
MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Na-nog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and alpha-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source.
The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.
间充质干细胞（MSCs）是再生医学中一种很有前景的工具，但由于其群体的异质性、不同的来源和分离技术，定义MSCs的特征并不一致。
本研究的目的是比较源自两种不同人体组织的MSCs的特征：外周血（PB-MSCs）和脐带华通氏胶（UC-MSCs）。
分别通过梯度密度分离后的贴壁法或组织块培养法分离PB-MSC和UC-MSC，并比较它们的形态、克隆形成效率、增殖率、免疫表型和分化潜能。
源自两种来源的MSCs表现出相似的形态、增殖能力和多谱系（成骨、软骨生成、脂肪生成和肌生成）分化潜能。在克隆形成能力和免疫表型方面观察到差异，因为UC-MSCs显示出更高的CFU-F（集落形成单位-成纤维细胞）克隆效率，以及更高的胚胎标志物（Nanog、Sox2、SSEA4）表达。当通过流式细胞术（CD44、CD90、CD105、CD33、CD34、CD45、CD11b、CD235a）或免疫荧光标记（波形蛋白、STRO-1和α-平滑肌肌动蛋白）分析其他表面抗原时，无论MSCs来源如何，大多数似乎都具有相似的表位谱。
获得的结果表明，两种MSCs都是可用于细胞治疗应用的成体MSCs的良好替代来源。
