Abouzeed Yousef M, Zgheel Faraj, Elfahem Abdurrezagh Abdalla, Almagarhe Mohammed Saad, Dhawi Abdulgader, Elbaz Abdulkareem, Hiblu Murad A, Kammon Abdulwahab, Ahmed Mohamed O
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Department of Microbiology, Biotechnology Research Centre, Tripoli, Libya.
Open Vet J. 2018;8(4):479-484. doi: 10.4314/ovj.v8i4.20. Epub 2018 Dec 26.
In this study, antibacterial, antioxidant and phenolic compounds of raisin extracts was evaluated. Different solvent extraction methods were utilized to extract phenolic compounds from raisin. The phenolic compounds of raisin extracts was determined using HPLC and three compounds were found, catechin, quercetin and rutin. The antibacterial activity of the acetonic extract was tested against four bacterial pathogens viz. and using both disk diffusion and minimum inhibitory concentration (MIC) methods. The acetonic extract exhibited the highest activity against Staphylococcus aureus with zone of inhibition of 14mm and the MIC of 25 mg/ml. However, there was a little activity against and . The methanolic extract showed good antioxidant activity as indicated by 2,2-diphenyl-1-picrylhydrazyl free radical assay (DPPH). In conclusion, raisin contains potent phenolic compounds and their application might function as promising natural preservative and antimicrobial agents in food industry and also good antioxidant food that helps to minimize the risk of degenerative diseases.
在本研究中，对葡萄干提取物的抗菌、抗氧化和酚类化合物进行了评估。采用不同的溶剂萃取方法从葡萄干中提取酚类化合物。使用高效液相色谱法测定葡萄干提取物中的酚类化合物，发现了三种化合物，即儿茶素、槲皮素和芦丁。采用纸片扩散法和最低抑菌浓度（MIC）法测试了丙酮提取物对四种细菌病原体的抗菌活性。丙酮提取物对金黄色葡萄球菌表现出最高活性，抑菌圈为14mm，最低抑菌浓度为25mg/ml。然而，对[此处原文缺失两种细菌名称]的活性较低。如2,2-二苯基-1-苦基肼自由基测定法（DPPH）所示，甲醇提取物表现出良好的抗氧化活性。总之，葡萄干含有强效酚类化合物，它们在食品工业中作为有前景的天然防腐剂和抗菌剂发挥作用，也是有助于降低退行性疾病风险的优质抗氧化食品。
