Metabolite Profiles of Raw Peanut Seeds Reveal Differences between Market-Types.

Peanuts (Arachis hypogaea L.) are prized for their flavor and popular worldwide as food or as food ingredients. The raw peanut seed contains the precursor compounds to roasted peanut flavor and has the potential to be manipulated through traditional breeding methods. However, little is known about the metabolome of the raw seeds. Comprehensive metabolite profiles of both raw runner and Virginia-type peanuts were determined. Using a system incorporating several methodologies including (RP)/UPLC-MS/MS and HILIC/UPLC-MS/MS, along with quantitation of fatty acids, free amino acids, and tocopherols, 365 metabolites were identified and of these, 52 were significantly different between market types (P < 0.05). Higher levels of gamma-glutamylalanine, oxylipins, purine metabolites, and alpha-ketoglutarate derived members of the glutamate family of amino acids defined the Virginia-type, while runner-type peanuts were differentiated by their ethylmalonate and eicosenoate content. This study presents a comprehensive analysis of the raw peanut seed, providing knowledge of the range of small molecules present in peanuts. The new information presented here will enable future research for peanut quality improvement. PRACTICAL APPLICATION: Peanuts are widely used as snack foods and as food ingredients. Knowledge of the secondary metabolite compounds in raw peanuts is needed to determine their importance in peanut flavor and nutritional quality. This report used a nontargeted analytical approach for the identification of these types of compounds in peanuts for the first time. These data were supplemented with quantitative analysis of free amino acids and tocopherols and discussed as potential flavor precursors and health promoting compounds.