Mandáková Terezie, Lysak Martin A
Central European Institute of Technology (CEITEC), Masaryk University, Czech Republic.
Curr Protoc Plant Biol. 2016 Mar;1(2):359-371. doi: 10.1002/cppb.20022.
Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described. © 2016 by John Wiley & Sons, Inc.
染色体描绘（CP）是指用染色体特异性DNA探针进行荧光原位杂交（FISH），以识别大的染色体区域、染色体臂和整条染色体。对于植物中的CP和CCP（比较染色体描绘），最常用的是来自原始物种或相关物种的染色体特异性细菌人工染色体（BAC）重叠群作为描绘探针。CP能够在所有有丝分裂和减数分裂阶段以及相应的间期染色体区域中可视化和追踪特定的染色体区域和/或染色体。CCP能够识别两个或更多物种之间共享的大规模同源染色体区域和染色体。本文描述了一种基于使用拟南芥染色体特异性BAC重叠群在拟南芥中进行CP以及在其他十字花科类群中进行CCP的分步方案。© 2016约翰威立国际出版公司
