Lysak M A, Mandáková T, Lacombe E
Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
Cytogenet Genome Res. 2010 Jul;129(1-3):184-9. doi: 10.1159/000312951. Epub 2010 May 26.
In crucifer cytogenomics, BAC contigs of Arabidopsis thaliana have been used as probes for comparative chromosome painting among species. Here we successfully tested chromosome-specific BAC contigs of A. thaliana (n = 5) and A. halleri (n = 8) as probes for reciprocal BAC painting. Furthermore, BAC contigs of both Arabidopsis species were applied as multi-species painting probes to a third crucifer species, Noccaea caerulescens (n = 7), revealing their shared chromosome homeology. Specifically, we found homeology across portions of chromosomes At2, Ah4, and Nc4, which reflects their shared common origin with chromosome AK4 of the Ancestral Crucifer Karyotype (n = 8). We argue that multi-species and multi-directional painting will significantly expedite comparative cytogenomics in Brassicaceae and other plant families.
在十字花科细胞基因组学中，拟南芥的细菌人工染色体（BAC）重叠群已被用作物种间比较染色体描绘的探针。在此，我们成功测试了拟南芥（n = 5）和盐芥（n = 8）的染色体特异性BAC重叠群作为相互BAC描绘的探针。此外，这两个拟南芥物种的BAC重叠群被用作多物种描绘探针，应用于第三个十字花科物种天蓝遏蓝菜（n = 7），揭示了它们共有的染色体同源性。具体而言，我们发现了拟南芥2号染色体、盐芥4号染色体和天蓝遏蓝菜4号染色体部分区域的同源性，这反映了它们与祖先十字花科核型（n = 8）的AK4染色体有着共同的起源。我们认为，多物种和多方向描绘将显著加快十字花科及其他植物科的比较细胞基因组学研究。
