Generation of Stable Transgenic Rice (Oryza sativa L.) by Agrobacterium-Mediated Transformation.

Rice (Oryza sativa L.) is the staple food for more than half of the world's population, and has also become an important model monocot. As a result, numerous genetic transformation protocols have been developed to improve and better understand this particular agronomic plant. Here we introduce a convenient transformation method using Agrobacterium. The explants used are embryogenic calli derived from mature seeds, which are easily obtained and can be used all year round. After selection and regeneration, transformants are obtained from resistant calli cultured on the regeneration medium. This protocol has been used to generate transgenic rice (Oryza sativa L.) in as little as 4 months. © 2016 by John Wiley & Sons, Inc.