Zhang Yi, Li Jun, Gao Caixia
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Curr Protoc Plant Biol. 2016 Mar;1(2):235-246. doi: 10.1002/cppb.20004.
Rice (Oryza sativa L.) is the staple food for more than half of the world's population, and has also become an important model monocot. As a result, numerous genetic transformation protocols have been developed to improve and better understand this particular agronomic plant. Here we introduce a convenient transformation method using Agrobacterium. The explants used are embryogenic calli derived from mature seeds, which are easily obtained and can be used all year round. After selection and regeneration, transformants are obtained from resistant calli cultured on the regeneration medium. This protocol has been used to generate transgenic rice (Oryza sativa L.) in as little as 4 months. © 2016 by John Wiley & Sons, Inc.
水稻（Oryza sativa L.）是世界上一半以上人口的主食，并且已成为一种重要的单子叶模式植物。因此，人们已开发出许多遗传转化方案来改良并更好地了解这种特殊的农作物。在此，我们介绍一种利用农杆菌的便捷转化方法。所用外植体是来自成熟种子的胚性愈伤组织，其易于获取且全年均可使用。经过筛选和再生，从在再生培养基上培养的抗性愈伤组织中获得转化体。该方案已用于在短短4个月内培育出转基因水稻（Oryza sativa L.）。© 2016约翰威立父子出版公司
