Lee Jae Hee, Lee Jong Ho
Clin Lab. 2019 Jan 1;65(1). doi: 10.7754/Clin.Lab.2018.180730.
The Allplex Respiratory Panels 1, 2, and 3 (Allplex; Seegene, Republic of Korea) is a one-step real-time reverse transcription-PCR method based on the multiple detection temperature (MuDT) technology for the detection of respiratory viral infections. In this study, we evaluated the performance of the Allplex assay by comparing it with that of the Anyplex II RV16 detection kit (Anyplex; Seegene), a multiplex real-time PCR assay based on the tagging oligonucleotide cleavage and extension technology.
A total of 400 clinical respiratory specimens that were previously tested by the Anyplex assay (300 samples that revealed positive results, and 100 samples that revealed negative results) were analyzed using the Allplex assay.
After comparing both assays for detecting each virus, the range of positive percent agreement, negative percent agreement, and kappa values were found to be 91.7% to 100%, 94.1% to 100%, and 0.659 to 1.000, respectively. The uniplex PCR and sequencing for the samples with discrepant results revealed that a majority of the results were concordant with the results from the Allplex assay. In addition, the Allplex assay was superior in detecting multiple viruses.
The Allplex assay produces results comparable to those of the Anyplex assay. Thus, the Allplex assay can be proposed as a rapid and accurate method for detecting respiratory viruses, especially for the detection of multiple viral infections.
Allplex呼吸道检测试剂盒1、2和3（Allplex；韩国Seegene公司）是一种基于多重检测温度（MuDT）技术的一步法实时逆转录聚合酶链反应（RT-PCR）方法，用于检测呼吸道病毒感染。在本研究中，我们通过将Allplex检测法与Anyplex II RV16检测试剂盒（Anyplex；韩国Seegene公司）进行比较，评估了Allplex检测法的性能，Anyplex II RV16检测试剂盒是一种基于标记寡核苷酸切割和延伸技术的多重实时PCR检测法。
使用Allplex检测法对400份先前经Anyplex检测法检测过的临床呼吸道标本进行分析（300份检测结果为阳性的样本和100份检测结果为阴性的样本）。
在比较两种检测法对每种病毒的检测结果后，发现阳性一致率、阴性一致率和kappa值的范围分别为91.7%至100%、94.1%至100%和0.659至1.000。对结果不一致的样本进行单重PCR和测序显示，大多数结果与Allplex检测法的结果一致。此外，Allplex检测法在检测多种病毒方面更具优势。
Allplex检测法产生的结果与Anyplex检测法相当。因此，Allplex检测法可被认为是一种快速、准确的呼吸道病毒检测方法，尤其是用于检测多种病毒感染。
