Hashimoto Y, Kato K, Sato N, Yagi H, Yagita H, Nishimura T
Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
Princess Takamatsu Symp. 1988;19:315-22.
Properties of mouse lymphokine activated-killer (LAK) cells were examined by using polyclonal and monoclonal LAK cells. To identify cell types of LAK cells, LAK cells were induced from various mouse lymphoid tissues and examined for the surface phenotypes by means of negative selection and FACS analysis. Irrespective of the cell sources, LAK cells expressed Thy 1 and lymphocyte function-associated antigen 1 (LFA-1) as common markers but the expressions of asialoGM1 and Lyt antigens were different from their cell sources. The induction of LAK cells from spleen cells was more frequent in asialoGM1+, Lyt 2- (natural killer (NK) cell type) cells than in asialoGM1-, Lyt 2+ (T cell type). To further assess the properties of LAK cells, we established LAK cell clones from LAK cell lines induced from C57BL/6 mouse spleen cells. Although these clones expressed the similar phenotypes to the parent LAK cells, Lyt 2 was expressed in a limited portion of the clones. All clones were found to express T3 and T cell receptor (TcR)-alpha beta, and rearrangement patterns of TcR-beta were the same among the clones derived from the same parent cell line but different in clones derived from different cell lines as determined by using C beta 1 and J beta 2 probes. The molecules responsible for LAK-target cell binding were examined by using killer blocking antibody (KBA) (anti-LFA-1) monoclonal antibody (mAb) and anti-idiotypic polyclonal antibody (Id) to KBA.(ABSTRACT TRUNCATED AT 250 WORDS)
利用多克隆和单克隆小鼠淋巴因子激活的杀伤细胞（LAK细胞）对其特性进行了检测。为了鉴定LAK细胞的细胞类型，从各种小鼠淋巴组织诱导出LAK细胞，并通过阴性选择和荧光激活细胞分选术（FACS）分析检测其表面表型。无论细胞来源如何，LAK细胞均表达Thy 1和淋巴细胞功能相关抗原1（LFA-1）作为共同标志物，但脱唾液酸GM1和Lyt抗原的表达因其细胞来源而异。从脾细胞诱导LAK细胞时，脱唾液酸GM1阳性、Lyt 2阴性（自然杀伤（NK）细胞类型）的细胞比脱唾液酸GM1阴性、Lyt 2阳性（T细胞类型）的细胞更频繁。为了进一步评估LAK细胞的特性，我们从C57BL/6小鼠脾细胞诱导的LAK细胞系中建立了LAK细胞克隆。尽管这些克隆表达的表型与亲本LAK细胞相似，但Lyt 2仅在有限部分的克隆中表达。所有克隆均表达T3和T细胞受体（TcR）αβ，并且通过使用Cβ1和Jβ2探针确定，来自同一亲本细胞系的克隆之间TcR-β的重排模式相同，但来自不同细胞系的克隆之间不同。通过使用杀伤阻断抗体（KBA）（抗LFA-1）单克隆抗体（mAb）和针对KBA的抗独特型多克隆抗体（Id）来检测负责LAK靶细胞结合的分子。（摘要截短于250字）
