Kato K, Sato N, Tanabe T, Yagita H, Agatsuma T, Hashimoto Y
Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University, Sendai.
Jpn J Cancer Res. 1991 Apr;82(4):456-63. doi: 10.1111/j.1349-7006.1991.tb01870.x.
To assess the properties of lymphokine-activated killer (LAK) cells, we established mouse LAK cell clones from LAK cell lines induced from C57BL/6 mouse spleen cells. Although these clones expressed similar phenotypes to the parent LAK cells, Lyt-2 was expressed in a restricted portion of the clones. All clones were found to express T3 CD2 and T cell receptor (TcR) alpha beta on their cell surface. Rearrangement patterns of TcR beta were the same among the clones derived from the same parent cell line but differed in those from different cell lines as determined by using C beta 1 and J beta 2 probes. The molecules responsible for LAK-target cell binding were examined by using a monoclonal antibody (mAb) against lymphocyte function associated antigen 1 (LFA-1). This mAb (termed KBA) showed inhibitory effects on both LAK-target cell binding and cytolytic activity of LAK cell clones, indicating a principal role of LFA-1 in LAK cell clones. The magnitude of perforin mRNA expression in LAK cell clones was unrelated to their cytolytic activities.
为了评估淋巴因子激活的杀伤（LAK）细胞的特性，我们从C57BL/6小鼠脾细胞诱导产生的LAK细胞系中建立了小鼠LAK细胞克隆。尽管这些克隆表现出与亲本LAK细胞相似的表型，但Lyt-2仅在部分克隆中表达。所有克隆在其细胞表面均表达T3、CD2和T细胞受体（TcR）αβ。通过使用Cβ1和Jβ2探针确定，源自同一亲本细胞系的克隆之间TcRβ的重排模式相同，但源自不同细胞系的克隆之间则不同。通过使用抗淋巴细胞功能相关抗原1（LFA-1）的单克隆抗体（mAb）来检测负责LAK-靶细胞结合的分子。该mAb（称为KBA）对LAK-靶细胞结合和LAK细胞克隆的细胞溶解活性均显示出抑制作用，表明LFA-1在LAK细胞克隆中起主要作用。LAK细胞克隆中穿孔素mRNA表达的水平与其细胞溶解活性无关。
