MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, SW7 2AZ, UK.
J Mol Biol. 2019 Sep 20;431(20):4078-4092. doi: 10.1016/j.jmb.2019.02.008. Epub 2019 Feb 15.
The parasitic life cycle of viruses involves the obligatory subversion of the host's macromolecular processes for efficient viral progeny production. Viruses that infect bacteria, bacteriophages (phages), are no exception and have evolved sophisticated ways to control essential biosynthetic machineries of their bacterial prey to benefit phage development. The xenogeneic regulation of bacterial cell function is a poorly understood area of bacteriology. The activity of the bacterial transcription machinery, the RNA polymerase (RNAP), is often regulated by a variety of mechanisms involving small phage-encoded proteins. In this review, we provide a brief overview of known phage proteins that interact with the bacterial RNAP and compare how two prototypical phages of Escherichia coli, T4 and T7, use small proteins to "puppeteer" the bacterial RNAP to ensure a successful infection.
病毒的寄生生命周期涉及对宿主大分子过程的强制性颠覆，以有效产生病毒后代。感染细菌的病毒，即噬菌体（phages），也不例外，它们已经进化出了复杂的方法来控制细菌猎物的必要生物合成机制，以促进噬菌体的发展。细菌细胞功能的异种调节是细菌学中一个了解甚少的领域。细菌转录机制，RNA 聚合酶（RNAP）的活性通常受到多种机制的调节，其中包括各种由小噬菌体编码的蛋白质。在这篇综述中，我们简要概述了已知与细菌 RNAP 相互作用的噬菌体蛋白，并比较了两种典型的大肠杆菌噬菌体 T4 和 T7 如何利用小蛋白“操纵”细菌 RNAP，以确保成功感染。
