a Research Center for Emerging Infections and Zoonoses (RIZ) , University of Veterinary Medicine Hannover (TiHo) , Hannover , Germany.
Expert Rev Vaccines. 2019 Apr;18(4):379-392. doi: 10.1080/14760584.2019.1582338. Epub 2019 Mar 4.
Various viruses, including poxviruses, adenoviruses and vesicular stomatitis virus, have been considered as vaccine vectors for the delivery of antigens of interest in the development of vaccines against newly emerging pathogens.
Here, we review results that have been obtained with influenza A viruses (IAV) as vaccine vectors. With the advent of reverse genetics technology, IAV-based recombinant vaccine candidates have been constructed that induce protective immunity to a variety of different pathogens of interest, including West Nile virus, Plasmodium falciparum and respiratory syncytial virus. The various cloning strategies to produce effective and attenuated, safe to use IAV-based viral vectors are discussed.
It was concluded that IAV-based vector system has several advantages and holds promise for further development.
多种病毒，包括痘病毒、腺病毒和水疱性口炎病毒，已被视为疫苗载体，用于在针对新出现病原体的疫苗开发中递呈感兴趣的抗原。
在此，我们回顾了甲型流感病毒（IAV）作为疫苗载体所获得的成果。随着反向遗传学技术的出现，构建了基于 IAV 的重组候选疫苗，这些疫苗能够诱导针对多种不同感兴趣病原体的保护性免疫，包括西尼罗河病毒、疟原虫和呼吸道合胞病毒。讨论了产生有效和减毒、安全使用的基于 IAV 的病毒载体的各种克隆策略。
结论认为，基于 IAV 的载体系统具有多种优势，具有进一步发展的前景。
