Akirin1 通过调节多种成肌细胞分化因子促进成肌细胞分化。
Akirin1 promotes myoblast differentiation by modulating multiple myoblast differentiation factors.
机构信息
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, P.R. China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, Sichuan, P.R. China
出版信息
Biosci Rep. 2019 Mar 1;39(3). doi: 10.1042/BSR20182152. Print 2019 Mar 29.
Akirin1 is found to be involved in myoblast differentiation. However, the mechanism by which the Akirin1 gene regulates myoblast differentiation still remains unclear. In the present study, we found that ectopic expression of Akirin1 promoted myoblast differentiation by increasing the expression of myogenic regulatory factor (MRF) 4 () and myocyte enhancer factor 2B () mRNA. Additionally, we showed that ectopic Akirin1 induced cell cycle arrest by up-regulating mRNA. To further uncover the mechanism by which Akirin1 promotes myoblast differentiation, we showed that the enhanced Akirin1 increased the mRNA expression of P38α. Importantly, the enhanced MRF4 expression by Akirin1 can be abrogated by treatment of SB203580, a p38 inhibitor. Similarly, we found that enhanced MEF2B expression by Akirin1 can be abrogated by treatment with LY294002, a PI3K inhibitor. Together, our results indicate that Akirin1 promotes myoblast differentiation by acting on the p38 and PI3K pathways and subsequently inducing the expression of myoblast differentiation factors.
Akirin1 被发现参与成肌细胞分化。然而，Akirin1 基因调节成肌细胞分化的机制仍不清楚。在本研究中，我们发现过表达 Akirin1 通过增加肌调节因子 (MRF) 4() 和肌细胞增强因子 2B()mRNA 的表达促进成肌细胞分化。此外，我们表明异位表达的 Akirin1 通过上调 mRNA 诱导细胞周期停滞。为了进一步揭示 Akirin1 促进成肌细胞分化的机制，我们表明增强的 Akirin1 增加了 P38α 的 mRNA 表达。重要的是，Akirin1 增强的 MRF4 表达可以通过 p38 抑制剂 SB203580 处理来消除。同样，我们发现 Akirin1 增强的 MEF2B 表达可以通过 PI3K 抑制剂 LY294002 处理来消除。总之，我们的结果表明，Akirin1 通过作用于 p38 和 PI3K 途径，随后诱导成肌细胞分化因子的表达，促进成肌细胞分化。
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