Rostamian Mosayeb, Akya Alisha, Niknam Hamid M
Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Immunology Department, Pasteur Institute of Iran, 69 Pasteur Avenue, Tehran, 13169-43551, Iran.
Parasitol Res. 2019 Apr;118(4):1231-1237. doi: 10.1007/s00436-019-06256-3. Epub 2019 Feb 19.
Humoral (antibody) response is an important part of immunity against pathogens. Despite the clear role of cell-mediated immune response in protection against leishmaniasis, the role of humoral responses is challenging. There is very limited data regarding humoral immune response against Leishmania tropica which is the causative agent of human cutaneous leishmaniasis in many parts of the world. Here, we have compared pathogenicity and antibody response against six Iranian Leishmania tropica isolates in BALB/c mice. A Leishmania major isolate was used for comparison. The parasites were injected into the mice followed by the evaluation of the lesion development, parasite load, and antibody responses (IgG1 and IgG2a). Our findings showed that some isolates caused the large lesions and high parasite load in the spleen and lymph node, while other isolates led to no lesion, no splenic parasitism, and low parasite load in the lymph node. The more pathogenic isolates induced higher antibody responses (IgG1 and IgG2a). Our results indicated that there is substantial heterogeneity among various Leishmania tropica isolates regarding the humoral immune response as well as the pathogenicity.
体液（抗体）反应是针对病原体免疫的重要组成部分。尽管细胞介导的免疫反应在预防利什曼病中发挥着明确作用，但体液反应的作用仍具有挑战性。关于针对热带利什曼原虫的体液免疫反应的数据非常有限，而热带利什曼原虫是世界许多地区人类皮肤利什曼病的病原体。在此，我们比较了BALB/c小鼠对六种伊朗热带利什曼原虫分离株的致病性和抗体反应。使用一株硕大利什曼原虫分离株作为对照。将寄生虫注射到小鼠体内，随后评估病变发展、寄生虫载量和抗体反应（IgG1和IgG2a）。我们的研究结果表明，一些分离株在脾脏和淋巴结中引起大的病变和高寄生虫载量，而其他分离株则未导致病变、脾脏无寄生现象且淋巴结中的寄生虫载量较低。致病性更强的分离株诱导产生更高的抗体反应（IgG1和IgG2a）。我们的结果表明，不同的热带利什曼原虫分离株在体液免疫反应以及致病性方面存在很大的异质性。
