Laboratory Medicine, People's Hospital of Rizhao, Rizhao, China.
Eur Rev Med Pharmacol Sci. 2019 Feb;23(3):1108-1115. doi: 10.26355/eurrev_201902_17001.
Non-small cell lung cancer (NSCLC) is the most common cause for cancer-related mortality worldwide. Currently, early detection of NSCLC is one of the main available strategies for improving its prognosis. Due to the lack of non-invasive and convenient tools, early diagnosis of NSCLC remains poor. Recently, it has been reported that circulating microRNAs (miRNAs) can be stably detected in serum. Meanwhile, they play a powerful role as biomarkers in various tumors. Therefore, the aim of this study was to detect the expression levels of serum miR-182, 200b and 205 in NSCLC patients, and to investigate their diagnostic and prognostic values.
Real-time quantitative polymerase chain reaction (RT-qPCR) was carried out to measure the expressions of miR-182, 200b and 205 in NSCLC tissues and normal controls. Receiver-operating characteristic (ROC) curve analysis was performed to assess the potential value of serum miRNAs for NSCLC diagnosis. Meanwhile, transwell assays were performed to observe the functional effects of miRNAs on the invasion and migration of NSCLC cells.
Compared with normal controls, serum levels of miR-182 and 205 in NSCLC patients were significantly upregulated, whereas miR-200b was remarkably downregulated. ROC analysis indicated that miRNA array (miR-182, 200b and 205) was useful biomarkers for early diagnosis of NSCLC. In addition, transwell assays demonstrated that miR-182 promoted the invasion and migration of NSCLC cells.
Our findings revealed that serum miR-182, 200b and 205 might serve as promising biomarkers for early detection and treatment of NSCLC.
非小细胞肺癌（NSCLC）是全球癌症相关死亡的最常见原因。目前，早期检测 NSCLC 是改善其预后的主要策略之一。由于缺乏非侵入性和方便的工具，NSCLC 的早期诊断仍然很差。最近，据报道，循环 microRNAs（miRNAs）可以在血清中稳定检测到。同时，它们在各种肿瘤中作为生物标志物发挥着强大的作用。因此，本研究旨在检测 NSCLC 患者血清 miR-182、200b 和 205 的表达水平，并探讨其诊断和预后价值。
采用实时定量聚合酶链反应（RT-qPCR）测量 NSCLC 组织和正常对照中 miR-182、200b 和 205 的表达。采用受试者工作特征（ROC）曲线分析评估血清 miRNAs 对 NSCLC 诊断的潜在价值。同时，进行 transwell 测定以观察 miRNAs 对 NSCLC 细胞侵袭和迁移的功能影响。
与正常对照组相比，NSCLC 患者血清 miR-182 和 205 水平显著上调，而 miR-200b 显著下调。ROC 分析表明，miRNA 阵列（miR-182、200b 和 205）是 NSCLC 早期诊断的有用生物标志物。此外，transwell 测定表明 miR-182 促进了 NSCLC 细胞的侵袭和迁移。
我们的研究结果表明，血清 miR-182、200b 和 205 可能作为 NSCLC 早期检测和治疗的有前途的生物标志物。
