Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Contact Dermatitis Investigation Unit, Salford Royal NHS Foundation Trust, Salford, UK.
Contact Dermatitis. 2019 Jul;81(1):43-53. doi: 10.1111/cod.13246. Epub 2019 Apr 8.
There is considerable interest in understanding the immunological variables that have the greatest influence on the effectiveness of sensitization by contact allergens, particularly in the context of developing new paradigms for risk assessment of novel compounds.
To examine the relationship between patch test score for three different contact allergens and the characteristics of T cell responses.
A total of 192 patients with confirmed nickel, p-phenylenediamine (PPD) or methylisothiazolinone (MI) allergy were recruited from the Contact Dermatitis Investigation Unit at Salford Royal Hospital. Severity of allergy was scored by the use of patch testing, peripheral blood lymphocytes were characterized for T cell phenotype by flow cytometry, and proliferative activity was characterized by radiolabelled thymidine incorporation. Comparisons were drawn with buffy coat samples from healthy volunteers.
Patch test positivity for nickel, PPD and MI was associated with changes in the phenotype of peripheral blood T cells: increases in naïve cells, decreases in regulatory T cell frequency and the CD4 /CD8 ratio, and increased expression of the skin-homing marker cutaneous lymphocyte antigen (CLA), particularly for those patients with a +++ patch test score.
This increased understanding of the characteristics of the T cell responses to contact allergens may provide parameters with which to better measure health risks associated with skin sensitization.
人们对理解影响接触过敏原致敏效果的免疫变量非常感兴趣，尤其是在为新型化合物开发风险评估新范式的背景下。
研究三种不同接触过敏原斑贴试验评分与 T 细胞反应特征之间的关系。
共招募了 192 名在索尔福德皇家医院接触性皮炎研究中心确诊的镍、对苯二胺 (PPD) 或甲基异噻唑啉酮 (MI) 过敏的患者。通过斑贴试验对过敏严重程度进行评分，通过流式细胞术对外周血淋巴细胞的 T 细胞表型进行特征分析，并通过放射性标记的胸苷掺入来描述增殖活性。并与健康志愿者的白细胞样本进行了比较。
镍、PPD 和 MI 的斑贴试验阳性与外周血 T 细胞表型的变化相关：幼稚细胞增加，调节性 T 细胞频率和 CD4/CD8 比值降低，皮肤归巢标志物皮肤淋巴细胞抗原 (CLA) 的表达增加，尤其是那些斑贴试验评分为 +++ 的患者。
对接触过敏原的 T 细胞反应特征的这种深入了解，可能为更好地衡量皮肤致敏相关的健康风险提供参数。
