Department of Physics, School of Mathematics and Physics, The University of Queensland, Brisbane, Queensland, Australia.
J Biophotonics. 2019 Jul;12(7):e201900022. doi: 10.1002/jbio.201900022. Epub 2019 Mar 20.
Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro and nano objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle's mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally. Here, we demonstrate that this system has the capacity to predict the hydrodynamic interaction between three-dimensional spatial membranes and internal probe particles within submicron distances, and it has the potential to aid in the design of high resolution optical micro/nanorheology techniques to be used inside living cells.
探索细胞内物质的流变特性对于理解细胞和亚细胞过程至关重要。光学陷阱已被广泛用于在流体中对微纳物体进行物理操纵，从而实现对生物系统的研究。然而，实验仍然具有挑战性，因为不清楚探针粒子的迁移率如何受到附近膜和细胞器的影响。我们使用脂质体（单层脂质囊泡）作为活细胞的简单仿生模型，结合使用光学镊子旋转的捕获粒子，从理论和实验两方面研究脂质体内部的力学和流变特性。在这里，我们证明该系统能够预测三维空间膜与亚微米距离内内部探针粒子之间的流体动力相互作用，并且有可能辅助设计用于活细胞内部的高分辨率光学微/纳米流变技术。
