Alaniz S, León M, Vicent A, García-Jiménez J, Abad-Campos P, Armengol J
Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022-Valencia, Spain.
Plant Dis. 2007 Sep;91(9):1187-1193. doi: 10.1094/PDIS-91-9-1187.
In this work, 82 Cylindrocarpon isolates associated with black foot disease of grapevines in Spain were studied by means of phenotypical characterization, DNA analyses, and pathogenicity tests. Partial sequences of the beta-tubulin (BT) gene, BT1, were amplified using primers BT1a and BT1b. A unique and conserved 52-bp insertion in the BT1 sequence, which is a specific marker for C. macrodidymum, was found in 56 of the isolates. The rest of the isolates (26) were identified as C. liriodendri. The BT phylogeny grouped all the isolates of each species into two well-supported clades. Phenotypical data were subjected to multivariate factorial analysis. According to this study, the isolates were clearly separated into two groups which were in agreement with BT species identification. C. macrodidymum isolates were differentiated from C. liriodendri by producing fewer conidia, presenting longer and wider macroconidia, and lower growth rate at 5 and 10°C. Selected isolates of each species inoculated onto rooted cuttings of grapevine rootstock cv. 110 R caused typical black foot disease symptoms. This is the first report of C. liriodendri and C. macrodidymum causing black foot disease of grapevines in Spain.
在这项研究中，通过表型特征分析、DNA分析和致病性测试，对西班牙82株与葡萄黑脚病相关的柱孢属分离株进行了研究。使用引物BT1a和BT1b扩增β-微管蛋白（BT）基因BT1的部分序列。在56株分离株中发现BT1序列中有一个独特且保守的52bp插入片段，这是大孢柱孢的特异性标记。其余分离株（26株）被鉴定为鹅掌楸柱孢。BT系统发育分析将每个物种的所有分离株分为两个得到充分支持的进化枝。对表型数据进行多变量因子分析。根据这项研究，分离株明显分为两组，这与BT物种鉴定结果一致。大孢柱孢分离株与鹅掌楸柱孢的区别在于产孢量较少、大型分生孢子更长更宽，以及在5℃和10℃下生长速率较低。将每个物种的选定分离株接种到葡萄砧木品种110R的生根插条上，会引起典型的黑脚病症状。这是西班牙首次报道鹅掌楸柱孢和大孢柱孢引起葡萄黑脚病。
