在佛罗里达州两家苗圃的蝴蝶兰中检测到凤仙花坏死斑点病毒和番茄斑萎病毒。

Impatiens necrotic spot virus and Tomato spotted wilt virus Diagnosed in Phalaenopsis Orchids from Two Florida Nurseries.

作者信息

Baker C A, Davison D, Jones L

机构信息

FDACS, Division of Plant Industry, Gainesville, FL 32614.

出版信息

Plant Dis. 2007 Nov;91(11):1515. doi: 10.1094/PDIS-91-11-1515A.

DOI:10.1094/PDIS-91-11-1515A

PMID:30780760

Abstract

In October 2006 (Arcadia, FL) and January 2007 (Sorrento, FL), several white Phalaenopsis orchids with large chlorotic/necrotic ringspot symptoms were sent to the Division of Plant Industry, Gainesville, FL. Symptomatic leaf tissues were tested with the Agdia immunostick-comb (Agdia, Elkhart, IN) for Impatiens necrotic spot virus (INSV), Tomato spotted wilt virus (TSWV), Cucumber mosaic, and Tobacco mosaic virus. Plants from the nursery in Sorrento, FL tested positive for TSWV, while those from the nursery in Arcadia, FL tested positive for INSV. Symptomless leaves from the infected plants tested negative for the viruses with the immunostick-comb. The plants also were tested for TSWV and INSV by double-antibody (DAS)-ELISA (Agdia Inc.) with the same results. Total RNA was extracted from one symptomatic orchid leaf from each nursery. Reverse transcription (RT)-PCR was performed with the universal tospovirus primer set BR60and BR65 (1). PCR bands of the expected size were amplified from each leaf. PCR products were sequenced directly. The orchid leaf that tested positive for TSWV by ELISA produced a 495-bp sequence with 97% identity to several isolates of the TSWV nucleocapsid protein gene listed in GenBank (Accession Nos. AY744479, AY8770391, DQ376185, and AF02659). The orchid leaf that tested positive for INSV by ELISA produced a 396-bp sequence with 98 to 99% identity to several isolates of the INSV nucleocapsid protein gene (Accession Nos. D00914, DQ425096, X66972, and AD109100). Although these viruses have been reported a few times in orchids previously (2,3), to our knowledge, this is the first time they have been reported in this host in Florida. In addition, white Phalaenopsis spp. appears to be a local lesion host and not a systemic host for these viruses. References: (1) M. Eiras et al. Fitopatol. Bras. 26:170, 2001. (2) J. S. Hu et al. Plant Dis. 77:464, 1993. (3) S. T. Koike and D. E. Mayhew. Orchids 70:746, 2001.

摘要

2006年10月（佛罗里达州阿卡迪亚）和2007年1月（佛罗里达州索伦托），几株带有大型褪绿/坏死环斑症状的白色蝴蝶兰被送到佛罗里达州盖恩斯维尔的植物产业部门。对有症状的叶片组织使用Agdia免疫检测条（Agdia公司，印第安纳州埃尔克哈特）检测凤仙花坏死斑病毒（INSV）、番茄斑萎病毒（TSWV）、黄瓜花叶病毒和烟草花叶病毒。佛罗里达州索伦托苗圃的植株检测出TSWV呈阳性，而佛罗里达州阿卡迪亚苗圃的植株检测出INSV呈阳性。用免疫检测条对受感染植株无症状的叶片进行病毒检测，结果呈阴性。还用双抗体（DAS）-ELISA（Agdia公司）对这些植株检测TSWV和INSV，结果相同。从每个苗圃的一株有症状的兰花叶片中提取总RNA。使用通用的番茄斑萎病毒引物对BR60和BR65进行逆转录（RT）-PCR（1）。从每片叶子中扩增出预期大小的PCR条带。对PCR产物直接进行测序。通过ELISA检测呈TSWV阳性的兰花叶片产生了一个495碱基对的序列，与GenBank中列出的几种TSWV核衣壳蛋白基因分离株有97%的同一性（登录号：AY744479、AY8770391、DQ376185和AF02659）。通过ELISA检测呈INSV阳性的兰花叶片产生了一个396碱基对的序列，与几种INSV核衣壳蛋白基因分离株有98%至99%的同一性（登录号：D00914、DQ425096、X66972和AD109100）。尽管这些病毒此前曾在兰花中被报道过几次（2,3），但据我们所知，这是它们首次在佛罗里达州的这种寄主植物中被报道。此外，白色蝴蝶兰属植物似乎是这些病毒的局部病斑寄主，而非系统寄主。参考文献：（1）M. Eiras等人，《巴西植物病理学》26:170，2001年。（2）J. S. Hu等人，《植物病害》77:464，1993年。（3）S. T. Koike和D. E. Mayhew，《兰花》70:746，2001年。