Department of Earth and Sea Science, Laboratory of Marine Biochemistry and Ecotoxicology, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy.
Marine Biology Institute, Consorzio Universitario della Provincia di Trapani, Via Barlotta 4, 91100 Trapani, Italy.
Int J Environ Res Public Health. 2019 Feb 18;16(4):588. doi: 10.3390/ijerph16040588.
In the present study, we evaluated the effects of different concentrations of the polybrominated diphenyl ethers (PBDEs) BDE-209, BDE-47 and BDE-99, on the vitality and oxidative stress of a HS-68 human cell culture exposed to the compounds for three days. The results showed that for this exposure time, only the highest concentrations produced a significant vitality reduction and oxidative stress induction ( < 0.05), measured as reactive oxygen species (ROS). Subsequently, in order to verify the effects of sub-lethal doses, cells were exposed for a longer time and data collected, after 12 and 20 days, to study ROS production and some molecular markers related to cell cycle and stress (p53, pRB, PARP, c-Jun and c-Fos), antioxidant status and proliferation (ERK, c-Jun and c-Fos), energy balance (NRF2, AMPK, HIF). Most of the biomarkers were influenced by the treatments, indicating that sub-lethal doses of PBDEs, for longer time, can enhance the production of ROS, altering the energetic metabolism, cell cycle and antioxidant balance, determining possible negative effects on the cell proliferation equilibrium.
在本研究中，我们评估了不同浓度的多溴二苯醚（PBDEs）BDE-209、BDE-47 和 BDE-99 对暴露于这些化合物 3 天的 HS-68 人细胞培养活力和氧化应激的影响。结果表明，对于这种暴露时间，只有最高浓度才会导致显著的活力降低和氧化应激诱导（<0.05），表现为活性氧（ROS）。随后，为了验证亚致死剂量的影响，细胞暴露时间更长，并在 12 和 20 天后收集数据，以研究 ROS 产生以及与细胞周期和应激（p53、pRB、PARP、c-Jun 和 c-Fos）、抗氧化状态和增殖（ERK、c-Jun 和 c-Fos）、能量平衡（NRF2、AMPK、HIF）相关的一些分子标志物。大多数生物标志物受到处理的影响，表明亚致死剂量的 PBDEs 暴露时间更长，会增强 ROS 的产生，改变能量代谢、细胞周期和抗氧化平衡，从而可能对细胞增殖平衡产生负面影响。
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