Institut Charles Sadron, Université de Strasbourg, CNRS UP22, F-67200, Strasbourg, France.
Institute of Physical Chemistry, RWTH Aachen University, D-52074, Aachen, Germany.
Sci Rep. 2019 Feb 19;9(1):2292. doi: 10.1038/s41598-019-38620-9.
The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (CE) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of micrometer-sized CE LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the CE bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
通过共焦激光扫描显微镜（CLSM）对非离子表面活性剂三乙二醇单正癸醚（CE）的荧光标记大单室囊泡（LUV）的层状到海绵相转变进行了原位研究。在 20°C 下制备了稳定的微米级 CE LUV 分散体，并迅速在接近层状到海绵相转变温度的不同温度下加热。通过层间和层内融合，观察到强烈波动的单个囊泡通过多次多层形态转变为微米级海绵相液滴，从而发生相变。通过闪烁噪声光谱和空间自相关函数对稳定波动膜进行定量图像分析，支持 CE 双层极低的弯曲刚度和横向张力。
