利用共聚焦激光扫描荧光显微镜检测愈伤组织胚的新型染料。

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy.

机构信息

Department of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Daugavpils, Latvia.

Department of Ecology, Daugavpils University, Institute of Life Sciences and Technology, Daugavpils, Latvia.

出版信息

Luminescence. 2019 May;34(3):353-359. doi: 10.1002/bio.3616. Epub 2019 Feb 19.

DOI:10.1002/bio.3616

PMID:30784170

Abstract

In the present study a new luminescent dye 3-N-(2-pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3-aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.

摘要

在本研究中，合成了一种新的发光染料 3-N-(2-吡咯烷乙酰胺基)苯并蒽酮（AZR）。在七种有机溶剂中对新型苯并蒽酮 3-氨基衍生物进行了光谱测量，显示出强荧光。在所制备的染料在植物愈伤组织培养中可视化的能力已在亚麻、红三叶草和紫花苜蓿上进行了测试，以确定胚胎。用 AZR 对愈伤组织细胞进行染色，然后利用共聚焦激光扫描荧光显微镜进行进一步分析。进行的实验表明，新合成的荧光染料 AZR 的可视化效果非常高，它在快速和相对廉价的愈伤组织胚胎诊断方面非常有效，这些胚胎由于体外培养的特异性而成为问题。