Galal A, Radwan Lamiaa M, Rezik Habiba H, Ayoub H
Poultry Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Poultry Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
J Therm Biol. 2019 Feb;80:113-118. doi: 10.1016/j.jtherbio.2018.12.018. Epub 2018 Dec 17.
The naked neck gene was introduced by crossbreeding into Egyptian breeds to improve body weight. Expression levels of HSP70 and CPT-1 were used to assess the heat tolerance of three Egyptian local breeds (Fayoumi, Dandarawi and Sinai) with and without the naked neck gene and under normal and heat stress conditions. There were two genotypes from each breed that had the same genetic origin (the naked neck and normal plumage genotypes). For each genotype, chicks were divided into two groups, a control group and a treated group. Chicks in the treated group were subjected to heat stress (40 °C) for four hours when they were between 3 and 5 days old. This treatment was associated with a highly significant increase in HSP70 and CPT-1 gene expression for the Dandarawi breed compared to the levels in the Fayoumi and Sinai breeds. Moreover, the introduction of the naked neck gene into these local breeds caused marked increases in CPT-1 gene expression, but these increases did not significantly differ among different naked neck genotypes. Therefore, it could be concluded that the Dandarawi breed exhibited the best heat tolerance, followed by the Sinai breed, whereas the Fayoumi breed was inferior in this respect. Furthermore, the naked neck gene improved heat tolerance by increasing HSP70 gene expression rather than only by reducing feather cover. The results obtained recommended using the Sinia naked neck chicken as a male line in commercial parent stock to produce broiler chicks adapted to the hot and warm climates.
通过杂交将裸颈基因引入埃及品种以提高体重。利用热休克蛋白70（HSP70）和肉碱棕榈酰转移酶-1（CPT-1）的表达水平，评估了三种有或没有裸颈基因的埃及本地品种（法尤米、丹达拉维和西奈）在正常和热应激条件下的耐热性。每个品种都有两种具有相同遗传起源的基因型（裸颈基因型和正常羽毛基因型）。对于每种基因型，雏鸡被分为两组，一组为对照组，一组为处理组。处理组的雏鸡在3至5日龄时接受4小时的热应激（40°C）处理。与法尤米和西奈品种相比，这种处理使丹达拉维品种的HSP70和CPT-1基因表达显著增加。此外，将裸颈基因引入这些本地品种导致CPT-1基因表达显著增加，但不同裸颈基因型之间的增加没有显著差异。因此，可以得出结论，丹达拉维品种表现出最佳的耐热性，其次是西奈品种，而法尤米品种在这方面较差。此外，裸颈基因通过增加HSP70基因表达而不是仅通过减少羽毛覆盖来提高耐热性。所得结果建议将西奈裸颈鸡用作商业亲本种群的父系，以生产适应炎热和温暖气候的肉鸡雏鸡。
