DeVries R E, Trigiano R N, Windham M T, Windham A S, Sorochan J C, Rinehart T A, Vargas J M
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville 37996-4560.
Department of Plant Sciences, University of Tennessee, Knoxville 37996-4561.
Plant Dis. 2008 Jan;92(1):83-90. doi: 10.1094/PDIS-92-1-0083.
Sclerotinia homoeocarpa is the causal agent of dollar spot disease that reduces the uniformity and aesthetic value of golf putting greens. Fungicide-resistant isolates of S. homoeocarpa were collected from putting greens at 10 locations across Tennessee and northern Mississippi. Genetic diversity among the 60 isolates was investigated using vegetative compatibility, conserved gene sequences, and amplified fragment length polymorphism (AFLP). Six tester strains were paired with Tennessee and northern Mississippi isolates on potato dextrose agar. Some of the 60 isolates were delineated into vegetative compatibility groups, but fungicide resistance could not be associated with a particular vegetative compatibility group. Genetic similarities of isolates at the vegetative compatibility level could be attributed to founder effects. Sequencing the regions of CAD, EF1-α, β-tubulin, and internal transcribed spacers revealed 100% homology among isolates. Capillary gel electrophoresis and analysis of AFLP fragments indicated 86 to 100% similarity between the isolates. Vegetative compatibility and molecular data indicate that the populations of the pathogen are clonal. Isolates did not cluster according to fungicide resistance during unweighted pair group with arithmetic means analysis, but did appear to cluster according to vegetative compatibility group and location. Although associations could not be made between molecular markers and fungicide resistance, links between vegetative compatibility and AFLP markers may provide a foundation from which other studies could be performed.
核盘菌是导致美元斑病的病原菌，该病会降低高尔夫果岭的平整度和美观价值。从田纳西州和密西西比州北部的10个果岭收集了对杀菌剂具有抗性的核盘菌分离株。使用营养体亲和性、保守基因序列和扩增片段长度多态性（AFLP）研究了60个分离株之间的遗传多样性。将6个测试菌株与来自田纳西州和密西西比州北部的分离株在马铃薯葡萄糖琼脂上配对。60个分离株中的一些被划分为营养体亲和群，但杀菌剂抗性与特定的营养体亲和群无关。分离株在营养体亲和水平上的遗传相似性可归因于奠基者效应。对CAD、EF1-α、β-微管蛋白和内转录间隔区区域进行测序，结果显示分离株之间具有100%的同源性。毛细管凝胶电泳和AFLP片段分析表明分离株之间的相似性为86%至100%。营养体亲和性和分子数据表明该病原菌群体是克隆性的。在非加权算术平均法分析中，分离株没有根据杀菌剂抗性聚类，但似乎根据营养体亲和群和位置聚类。虽然分子标记与杀菌剂抗性之间没有关联，但营养体亲和性与AFLP标记之间的联系可能为其他研究提供基础。
