一种制备共价闭合环状质粒DNA的简单方法。
A simple method for the preparation of the covalently closed circular form of plasmid DNA.
作者信息
Le Brun J J, Rentier-Delrue F, Mercier L
机构信息
Dept. of Botany, University of Maryland, College Park 20742.
出版信息
Biotechniques. 1988 Oct;6(9):834, 837-8.
We describe a simple, rapid, inexpensive method for isolation of covalently closed circular plasmid DNA. The method involves the electrophoresis of crude DNA preparations in an agarose gel, electrotransfer onto a dialysis membrane and elution of the highly purified circular covalently closed plasmid DNA. Native and recombinant plasmid DNA have been purified by this method and shown to be suitable for restriction enzyme digestion and transformation of bacteria. The yield of this rapid purification procedure makes it a good alternative method to standard centrifugation in cesium chloride ethidium bromide gradients.
我们描述了一种简单、快速、廉价的共价闭合环状质粒DNA分离方法。该方法包括将粗制DNA制剂在琼脂糖凝胶中进行电泳，电转移至透析膜上，然后洗脱高度纯化的共价闭合环状质粒DNA。天然质粒DNA和重组质粒DNA均已通过此方法纯化，并显示适用于限制性内切酶消化和细菌转化。这种快速纯化方法的产量使其成为氯化铯溴化乙锭梯度标准离心法的良好替代方法。
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