Orberg P K, Sandine W E
Appl Environ Microbiol. 1984 Apr;47(4):677-80. doi: 10.1128/aem.47.4.677-680.1984.
A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.
通过将短时间的溶菌酶-变溶菌素细胞壁消化与麦克马斯特等人（《分析生物化学》109:47 - 54，1980年）对大肠杆菌质粒分离程序的改进相结合，开发了一种利用微升量试剂从乳酸链球菌中快速纯化质粒DNA的方法。所获得的制备物高度富集共价闭合环状DNA，该方法适用于至少40兆道尔顿的质粒。不需要在氯化铯-溴化乙锭密度梯度中进行离心。
