Aliaga Fandino Ana Cecilia, Kim Hyoujin, Rademaker Jesse David, Lee Ji-Young
School of Biological Sciences, College of Natural Science, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Department of Behavioural Biology, University Utrecht, Padualaan 8, Utrecht 3584CH, The Netherlands.
Biol Open. 2019 Mar 12;8(3):bio039677. doi: 10.1242/bio.039677.
Cambium contains a stem cell population that produces xylem and phloem tissues in a radial direction during the secondary growth stage. The growth of many storage roots, including in the radish, L., also depends on cambium. Interestingly, we observed numerous adventitious roots (ARs) emerging from the cambia of cut surfaces when the bases of radish storage tap roots were removed. Previous studies in Arabidopsis showed that the pathway regulates AR initiation and meristem establishment in an auxin-dependent manner Here, we provide evidence indicating the evolutionary conservation of the pathway during the AR development in radishes. Additionally, we found that expression of two cambium regulators, and , is induced in the cambium regions that are connected to emerging ARs via vascularization. Both AR formation and genes associated with this were induced by exogenous auxin. Our research suggests that some key cambium regulators might be reprogrammed to aid in the AR development in concert with the pathway.This article has an associated First Person interview with the first author of the paper.
形成层包含一个干细胞群体，在次生生长阶段，该群体沿径向产生木质部和韧皮部组织。许多贮藏根的生长，包括萝卜（Raphanus sativus L.）的贮藏根，也依赖于形成层。有趣的是，当去除萝卜贮藏主根的基部时，我们观察到许多不定根从切面的形成层中长出。先前在拟南芥中的研究表明，该途径以生长素依赖的方式调节不定根的起始和分生组织的建立。在这里，我们提供证据表明该途径在萝卜不定根发育过程中具有进化保守性。此外，我们发现两个形成层调节因子RSHB1和RSHB2的表达在通过维管化与正在形成的不定根相连的形成层区域中被诱导。不定根的形成以及与之相关的基因都受到外源生长素的诱导。我们的研究表明，一些关键的形成层调节因子可能会被重新编程，以与该途径协同作用来促进不定根的发育。本文有对该论文第一作者的相关第一人称访谈。
