Microbial Pathogens Associated with Proximal Dental Caries in the Primary Dentition and Their Association with Periodontal Disease in Children.

BACKGROUND

Certain specific bacterial species from the subgingival biofilm have demonstrated etiological relevance in the initiation and progression of periodontitis. Among all the bacteria studied, three have shown the highest association with proximal caries and bone loss: (Fn), (Ca), and (Cr). Therefore, the relevance of having accurate microbiological diagnostic techniques for their identification and quantification is clearly justified.

AIM

To identify the bacterial pathogens with alveolar bone loss and proximal caries in primary dentition and their association with periodontal disease utilizing deoxyribonucleic acid (DNA) microbial probe testing.

MATERIALS AND METHODS

Subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Fn, Ca, Cr, , and several other periodontopathogens were determined by DNA microbial probe testing method.

RESULTS

Samples detecting a high bacterial load of Fn, Ca and Cr in children having proximal caries associated with periodontal disease compared to children having proximal caries without periodontal disease ( ≤ 0.01).

CONCLUSIONS

Results suggested that there was a relationship between microbial pathogens associated with proximal dental caries in the primary dentition and periodontal disease in children. In addition, DNA microbial probe testing technology clearly analyzed the different loads of periodontopathogens in children who had with proximal caries associated with bone loss and is useful in microbial diagnostics for patients in dental practices.