Renauld A, Von Lawzewitsch I, Sverdlik R C, Pérez R L, Rodríguez R R, Foglia V G
Departamento de Fisiología, Facultad de Medicina, Buenos Aires, Argentina.
Acta Physiol Pharmacol Latinoam. 1988;38(3):345-61.
Mongrel, male, fasted, unanesthetized dogs under the following alternative treatments: 1) nil, 2) orchidectomy 4 months before the study, 3) orchidectomy 10 months in advance, 4) orchidectomy like in (3) followed by i.m. propylenglycol treatment, 0.05 ml/kg body wt./day, 15 days (vehicle controls, and 5) testosterone hemisuccinate in propylenglycol, 0.75 mg in 0.05 ml/kg body wt./day, for 15 days, were used in this study. Pancreas slices of animals of every group were stained with immunoperoxidase. The animals were fasted, anesthetized for pancreas removal. Pancreatic section mean absorbance was estimated in a Zeiss cytospectrophotometer with a coupled computer. Blood sugar (BS) and both serum, immunoreactive insulin (IRI) and free fatty acids (FFA) were assayed in these.
杂种雄性犬，禁食，未麻醉，接受以下不同处理：1）无处理；2）在研究前4个月进行睾丸切除术；3）提前10个月进行睾丸切除术；4）如（3）所述进行睾丸切除术后，每天肌肉注射丙二醇，剂量为0.05 ml/kg体重，共15天（溶剂对照组）；5）每天肌肉注射丙二醇溶解的睾酮半琥珀酸酯，剂量为0.75 mg/0.05 ml/kg体重，共15天。本研究使用了每组动物的胰腺切片进行免疫过氧化物酶染色。动物禁食后麻醉，用于切除胰腺。在配备计算机的蔡司细胞分光光度计上估计胰腺切片的平均吸光度。检测这些动物的血糖（BS）、血清免疫反应性胰岛素（IRI）和游离脂肪酸（FFA）。
