Corbett-Detig Russell, Medina Paloma, Frérot Hélène, Blassiau Christelle, Castric Vincent
Genomics Institute and Department of Biomolecular Engineering UC Santa Cruz Santa Cruz California 95064.
Université de Lille CNRS UMR 8198-Evo-Eco-Paleo F-59000 Lille France.
Evol Lett. 2019 Jan 30;3(1):93-103. doi: 10.1002/evl3.96. eCollection 2019 Feb.
Genes that do not segregate in heterozygotes at Mendelian ratios are a potentially important evolutionary force in natural populations. Although the impacts of segregation distortion are widely appreciated, we have little quantitative understanding about how often these loci arise and fix within lineages. Here, we develop a statistical approach for detecting segregation distorting genes from the comprehensive comparison of whole genome sequence data obtained from bulk gamete versus somatic tissues. Our approach enables estimation of map positions and confidence intervals, and quantification of effect sizes of segregation distorters. We apply our method to the pollen of two interspecific F1 hybrids of and and we identify three loci across eight chromosomes showing significant evidence of segregation distortion in both pollen samples. Based on this, we estimate that novel segregation distortion elements evolve and achieve high frequencies within lineages at a rate of approximately one per 244,000 years. Furthermore, we estimate that haploid-acting segregation distortion may contribute between 10% and 30% of reduced pollen viability in F1 individuals. Our results indicate haploid acting factors evolve rapidly and dramatically influence segregation in F1 hybrid individuals.
在杂合子中不以孟德尔比率分离的基因在自然种群中是一种潜在的重要进化力量。尽管分离畸变的影响已广为人知，但我们对这些基因座在谱系中出现和固定的频率仍缺乏定量的了解。在这里，我们开发了一种统计方法，通过对从大量配子与体细胞组织获得的全基因组序列数据进行全面比较来检测分离畸变基因。我们的方法能够估计图谱位置和置信区间，并量化分离畸变者的效应大小。我们将我们的方法应用于两个种间F1杂种的花粉，并在八条染色体上鉴定出三个位点，在两个花粉样本中均显示出明显的分离畸变证据。基于此，我们估计新的分离畸变元件在谱系中以大约每244,000年一个的速率进化并达到高频率。此外，我们估计单倍体作用的分离畸变可能导致F1个体中花粉活力降低的10%至30%。我们的结果表明，单倍体作用因子进化迅速，并对F1杂种个体的分离产生显著影响。
