Department of Forest Science, University of Alberta, T6G 2H1, Edmonton, Alberta, Canada.
Theor Appl Genet. 1984 Dec;69(2):145-51. doi: 10.1007/BF00272888.
Segregation ratios of allozymes in haploid female gametophytes obtained from viable seed were studied in a natural population of jack pine, Pinus banksiana. Stability of these ratios was assessed for three levels of the sexually reproductive crown as well as for four years of natural fertilization. Analyses of observed segregation ratios of four of five polymorphic isozyme loci showed good correspondence to the overall 1∶1 ratios expected for simple Mendelian inheritance. Allozymes of glucose-6-phosphate dehydrogenase did not segregate in the expected 1∶1 ratio. In addition, there were significant deviations from the expected segregation ratio for all the loci at some sampling positions on individual trees. Heterogeneity of segregation among trees, strata and years could be the result of pollen pool heterogeneity, segregation distortion and/or recessive lethal and semi-lethal gene combinations resulting in early embryo abortion. These types of segregation deviations in viable seed can affect the estimation of allele frequencies from bulked samples of a small number of individuals, the inference of heterozygosity/homozygosity of parental trees, and estimates of selfing rates.
在天然的短叶松种群中，研究了从有活力种子中获得的单倍体雌配子体的等位基因分离比。对三个有性生殖冠层水平以及四年自然受精的稳定性进行了评估。对五个多态同工酶位点中的四个位点的观察到的分离比的分析表明，与简单孟德尔遗传预期的 1:1 比例非常吻合。葡萄糖-6-磷酸脱氢酶的等位基因没有按预期的 1:1 比例分离。此外，在个体树上的一些采样位置，所有位点的分离比都存在显著偏离预期值的情况。树木、层次和年份之间分离的异质性可能是花粉池异质性、分离扭曲以及/或隐性致死和半致死基因组合导致早期胚胎流产的结果。这种在有活力的种子中的分离偏差会影响从少数个体的大量样本中估计等位基因频率、推断亲本树木的杂合性/纯合性，以及估计自交率。
