Cherkashova E A, Burunova V V, Bukharova T B, Namestnikova D D, Gubskii I L, Salikhova D I, Galitsina E V, Leonov G E, Chekhonin V P, Gubskii L V, Kisevev S L, Goldstein D V, Yarygin K N
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Bull Exp Biol Med. 2019 Feb;166(4):558-566. doi: 10.1007/s10517-019-04392-5. Epub 2019 Feb 20.
We compared the effects of placental mesenchymal stromal cells and neural progenitor cells derived from induced human pluripotent cells after their intravenous administration to rats in 24 h after transitory occlusion of the middle cerebral artery. The therapeutic effects were evaluated by the dynamics of animal survival, body weight, neurological deficit, and the volume of infarction focus in 7, 14, 30, and 60 days after surgery. Intravenous injection of neural progenitor cells produced a therapeutic effect on the course of experimental ischemic stroke by increasing animal survival in the most acute period and accelerating compensation of neurological deficit and body weight recovery. Neural progenitor cells were more effective than mesenchymal stromal cells from human placenta. The effectiveness of intravenous transplantation of neural progenitor cells in the model of occlusion of the middle cerebral artery is shown by us for the first time, although the therapeutic effect of their direct transplantation into the brain has already been described.
我们比较了人诱导多能干细胞来源的胎盘间充质基质细胞和神经祖细胞在大脑中动脉短暂闭塞24小时后静脉注射给大鼠的效果。通过观察术后7天、14天、30天和60天动物的存活情况、体重、神经功能缺损以及梗死灶体积的动态变化来评估治疗效果。静脉注射神经祖细胞对实验性缺血性中风病程产生了治疗作用，表现为在最急性期提高了动物存活率，并加速了神经功能缺损的代偿和体重恢复。神经祖细胞比人胎盘间充质基质细胞更有效。我们首次证明了神经祖细胞静脉移植在大脑中动脉闭塞模型中的有效性，尽管其直接移植到大脑中的治疗效果已经有过描述。
