Zhang Ling-Juan
School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.
Department of Dermatology, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Methods Mol Biol. 2019;1940:205-215. doi: 10.1007/978-1-4939-9086-3_15.
Epidermis, the outermost layer of the skin, plays a critical role as both a physical and immunological barrier protecting the internal tissues from external environmental insults, such as pathogenic bacteria, fungi, viruses, UV irradiation, and water loss. Epidermal keratinocytes (KC), the predominant cell type in the skin epidermis, are in the front line of skin defense. Here we describe methods to isolate and culture primary epidermal KC from neonatal and adult mouse skin and describe in vitro assays to study and characterize KC proliferation and differentiation and pro-inflammatory responses to viral products and UVB irradiation. These methods will be useful for researchers in the field of epidermal biology to set up in vitro assays to study the barrier and pro-inflammatory function of epidermal keratinocytes.
表皮是皮肤的最外层，作为物理和免疫屏障发挥着关键作用，保护内部组织免受外部环境侵害，如病原菌、真菌、病毒、紫外线辐射和水分流失。表皮角质形成细胞（KC）是皮肤表皮中的主要细胞类型，处于皮肤防御的第一线。在这里，我们描述了从新生和成年小鼠皮肤中分离和培养原代表皮KC的方法，并描述了用于研究和表征KC增殖、分化以及对病毒产物和UVB辐射的促炎反应的体外试验。这些方法将有助于表皮生物学领域的研究人员建立体外试验，以研究表皮角质形成细胞的屏障和促炎功能。
