硫胺素与电鳐和青蛙终板中烟碱型乙酰胆碱受体的结合。

Binding of thiamine to nicotinic acetylcholine receptor in torpedo marmorata and the frog end plate.

作者信息

Waldenlind L, Elfman L, Rydqvist B

出版信息

Acta Physiol Scand. 1978 Jun;103(2):154-59. doi: 10.1111/j.1748-1716.1978.tb06202.x.

DOI:10.1111/j.1748-1716.1978.tb06202.x

Abstract

Thiamine was found to bind to the isolated nicotinic receptor (nAChR) from Torpedo marmorata (KD = 3--5 X 10(-5) M). The binding was reversible and inhibited by alpha-neurotoxin from Naja naja siamensis. Acetylcholine binding was not inhibited by thiamine. When thiamine (5 X 10(-4) M) was applied to frog sartorius muscles a decrease of miniature end plate potential amplitudes was seen. This effect was readily reversible after the perfusion fluid of the muscle was changed to normal Ringer solution. Thus both biological and electrophysiological studies indicated a binding of thiamine to the nAChR.

摘要

已发现硫胺素可与电鳐（Torpedo marmorata）分离出的烟碱型受体（nAChR）结合（解离常数KD = 3 - 5×10⁻⁵ M）。这种结合是可逆的，并且被眼镜蛇（Naja naja siamensis）的α-神经毒素所抑制。硫胺素不会抑制乙酰胆碱的结合。当将硫胺素（5×10⁻⁴ M）应用于青蛙缝匠肌时，可观察到微小终板电位幅度降低。在将肌肉的灌注液换成正常林格氏液后，这种效应很容易逆转。因此，生物学和电生理学研究均表明硫胺素与nAChR存在结合。