Laboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agriculture Development, Democritus University of Thrace, 68200, Orestiada, Greece.
Food Biotechnology Group, Department of Chemistry, University of Patras, GR-26504, Patras, Greece.
Appl Biochem Biotechnol. 2019 Aug;188(4):1096-1107. doi: 10.1007/s12010-019-02977-4. Epub 2019 Feb 21.
A probiotic biocatalyst was prepared through Lactobacillus plantarum ATCC 14917 immobilization on a prebiotic carrier (delignified wheat bran) and was used for fermentations of pomegranate juice. Initially, pomegranate juice was fermented for 24 h and then was stored for 28 days at 4 °C. The obtained results regarding sugar and organic acid analysis revealed that the probiotic biocatalyst was effective. Ethanol was produced in small amounts (0.4-1% v/v). Total phenolic content and antioxidant activity was greater in the fermented pomegranate juice than in unfermented juice after 24 h of fermentation and over the time span of 28 days. Viability of probiotic cells was well maintained (above 8.65 log cfu/mL) after 24 h of fermentation and during 4 weeks of storage at 4 °C, and it is noteworthy that no pathogens were observed. The strength of viability of probiotic cells can be attributed to the immobilization carrier (delignified wheat bran) that exhibits prebiotic properties providing a protective effect to the cells. Finally, the proposed bioprocess of employing the proposed synbiotic biocatalyst for pomegranate juice fermentation shows great potential for commercialization while sensory evaluation highlights the degree of quality of the produced functional pomegranate beverages.
通过将植物乳杆菌 ATCC 14917 固定在益生元载体（脱木质化麦麸）上，制备了一种益生菌生物催化剂，并将其用于石榴汁发酵。最初，石榴汁发酵 24 小时，然后在 4°C 下储存 28 天。糖和有机酸分析的结果表明，益生菌生物催化剂是有效的。仅产生少量乙醇（0.4-1%v/v）。与未经发酵的石榴汁相比，发酵 24 小时后和 28 天的时间内，发酵石榴汁中的总酚含量和抗氧化活性更高。发酵 24 小时后和在 4°C 下储存 4 周期间，益生菌细胞的存活率保持良好（超过 8.65 log cfu/mL），值得注意的是，没有观察到病原体。益生菌细胞的活力强归因于固定化载体（脱木质化麦麸）具有益生元特性，为细胞提供了保护作用。最后，拟议的使用拟益生菌生物催化剂进行石榴汁发酵的生物工艺具有很大的商业化潜力，而感官评价则突出了所生产功能性石榴饮料的质量程度。
