酵母细胞内固醇转运的测量

Measurement of Intracellular Sterol Transport in Yeast.

作者信息

Chauhan Neha, Jentsch Julian A, Menon Anant K

机构信息

Department of Biochemistry, Weill Cornell Medical College, New York, NY, USA.

Institute for Pathophysiology, West German Heart and Vascular Centre, University Hospital Essen, Essen, Germany.

出版信息

Methods Mol Biol. 2019;1949:115-136. doi: 10.1007/978-1-4939-9136-5_10.

DOI:10.1007/978-1-4939-9136-5_10

PMID:30790253

Abstract

Intracellular sterol transport occurs largely by non-vesicular mechanisms in which sterol transport proteins extract sterol from one membrane and transfer it to another across the cytoplasm. Here we describe a suite of complementary assays to measure intracellular sterol transport in the model eukaryote Saccharomyces cerevisiae, as well as to quantify protein-mediated sterol transport between populations of vesicles in vitro. The in vivo assays can be adapted to study sterol transport in other cell types.

摘要

细胞内固醇转运主要通过非囊泡机制进行，在该机制中，固醇转运蛋白从一个膜中提取固醇，并将其穿过细胞质转移到另一个膜上。在此，我们描述了一套互补的检测方法，用于测量模式真核生物酿酒酵母中的细胞内固醇转运，以及在体外定量囊泡群体之间蛋白质介导的固醇转运。体内检测方法可用于研究其他细胞类型中的固醇转运。